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Simultaneous immersion Mirau interferometry
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10.1063/1.4803181
/content/aip/journal/rsi/84/5/10.1063/1.4803181
http://aip.metastore.ingenta.com/content/aip/journal/rsi/84/5/10.1063/1.4803181
View: Figures

Figures

Image of FIG. 1.
FIG. 1.

Configuration of the RARAF microbeam endstation.

Image of FIG. 2.
FIG. 2.

Schematics of immersion Mirau interferometric attachment.

Image of FIG. 3.
FIG. 3.

Components of SIMI: The light from a light source (LS) passes through a collimator lens (CL), a linear polarizer (P), and a band-pass filter (F) is reflected by a 50/50 beam-splitter (BS) and directed to the objective (O) with mounted SIMI interferometer (M). The interferometric attachment fits into the microbeam Petri dish (D). A λ/8 waveplate, placed in the test arm of the interferometer, introduces a quarter-wave phase shift to one component of the test beam (inset). Successive application of a polarization beam-splitter (PBS), that follows a tube lens (TL), enables separation of two interference patterns with different phase that are recorded on camera sensors (C1, C2). The arrows denote the directions of polarization.

Image of FIG. 4.
FIG. 4.

Design drawing of immersion Mirau interferometric attachment: 1—attachment body, 2—optics holders, 3—beam-splitter, 4—mirror, 5—adjustment screw, and 6—spring.

Image of FIG. 5.
FIG. 5.

Modifications of the camera adaptor of the SIMI microscope module: (a) adaptor for one camera with a polarization beam-displacer fitted inside; (b) adaptor for two cameras containing a polarization beam-splitter.

Image of FIG. 6.
FIG. 6.

SIMI images of live cells. (a) 3T3 cells were plated on mica substrates, illumination wavelength 540 nm; (b) WI 38 cells were plated on glass slides; illumination wavelength 605 nm. In both cases the cells were cultured in cell growth medium and imaged in PBS; to create each image, two interference patterns were formed on a single camera sensor. (c) HT 1080 cells plated on glass slides; illumination wavelength 540 nm. The cells were imaged in clear medium using two camera sensors.

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/content/aip/journal/rsi/84/5/10.1063/1.4803181
2013-05-03
2014-04-16
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Simultaneous immersion Mirau interferometry
http://aip.metastore.ingenta.com/content/aip/journal/rsi/84/5/10.1063/1.4803181
10.1063/1.4803181
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