Setup overview. (A) Controlled environment chamber, (B) 3D manipulator for removal capillary, (C) 3D manipulator for deposition capillary, (D) electromagnetic solenoid plunger, (E) syringe pump, (F) solid state relays, (G) National Instruments DAQs, and (H) computer with LabVIEW program.
Components within the controlled environment chamber. (A) Thermocouple, (B) humidity sensor, (C) cartridge heater, (D) vapor inlet, (E) tweezers, (F) copper grid, (G) removal capillary (filled with dark dye for contrast), and (H) hatch for plunging sample into cryogen. Deposition capillary (not shown) enters through the far wall.
Capillary positioning for sample deposition and removal for (a) time insensitive study and (b) time sensitive study. Solid arrows portray liquid motion and dashed arrows portray capillary motion. Conventional blotting entails the placement of the sample grid between two pieces of blotting paper (c), which are then pressed against the sample grid for sample thinning (d).
Wormlike micelles prepared using (a) our platform and (b) FEI Vitrobot. (a) Entangled wormlike micelles result from our sample preparation method of horizontal capillary action. (b) Parallel micelles result from blotting using the FEI Vitrobot. Scale bars are 200 nm.
Time-resolved cryo-TEM images of calcium carbonate solutions prepared with our platform after reaction times of (a) ∼700 ms and (b) 5 s. Scale bars are 200 nm.
Cryo-TEM image of Ultra Downy fabric softener demonstrates the feasibility of capillary action to sufficiently thin high viscosity fluid samples. Scale bar is 200 nm.
Cryo-TEM images of 0.0075 wt.% carbon black solutions prepared with (a) our method and (b) blotting. Scale bars are 500 nm.
(a) and (b) Finite element method simulations of shear within a 0.6 μm pore of the lacey support film of a copper TEM grid under a flow velocity of 10−3 m s−1. Streamlines are illustrated in white. (a) Conventional blotting results in high shear along the sides of the pore. (b) Horizontal capillary action results in orders of magnitude less shear within the pore. (c) and (d) Average shear along the horizontal centerline of a lacey support film pore for (c) blotting and (d) capillary action. Fluid velocities spanning three orders of magnitude and pore widths varying from 0.2 to 6 μm are modeled.
Article metrics loading...
Full text loading...