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/content/aip/journal/rsi/86/12/10.1063/1.4936843
1.
1.H. N. Chapman, P. Fromme, A. Barty, T. A. White, R. A. Kirian, A. Aquila, M. S. Hunter, J. Schulz, D. P. Deponte, U. Weierstall, R. B. Doak, F. R. N. C. Maia, A. V. Martin, I. Schlichting, L. Lomb, N. Coppola, R. L. Shoeman, S. W. Epp, R. Hartmann, D. Rolles, A. Rudenko, L. Foucar, N. Kimmel, G. Weidenspointner, P. Holl, M. N. Liang, M. Barthelmess, C. Caleman, S. Boutet, M. J. Bogan, J. Krzywinski, C. Bostedt, S. Bajt, L. Gumprecht, B. Rudek, B. Erk, C. Schmidt, A. Homke, C. Reich, D. Pietschner, L. Struder, G. Hauser, H. Gorke, J. Ullrich, S. Herrmann, G. Schaller, F. Schopper, H. Soltau, K. U. Kuhnel, M. Messerschmidt, J. D. Bozek, S. P. Hau-Riege, M. Frank, C. Y. Hampton, R. G. Sierra, D. Starodub, G. J. Williams, J. Hajdu, N. Timneanu, M. M. Seibert, J. Andreasson, A. Rocker, O. Jonsson, M. Svenda, S. Stern, K. Nass, R. Andritschke, C. D. Schroter, F. Krasniqi, M. Bott, K. E. Schmidt, X. Y. Wang, I. Grotjohann, J. M. Holton, T. R. M. Barends, R. Neutze, S. Marchesini, R. Fromme, S. Schorb, D. Rupp, M. Adolph, T. Gorkhover, I. Andersson, H. Hirsemann, G. Potdevin, H. Graafsma, B. Nilsson, and J. C. H. Spence, “Femtosecond X-ray protein nanocrystallography,” Nature 470, 73 (2011).
http://dx.doi.org/10.1038/nature09750
2.
2.R. Neutze, R. Wouts, D. van der Spoel, E. Weckert, and J. Hajdu, “Potential for biomolecular imaging with femtosecond X-ray pulses,” Nature 406, 752 (2000).
http://dx.doi.org/10.1038/35021099
3.
3.A. Barty, C. Caleman, A. Aquila, N. Timneanu, L. Lomb, T. A. White, J. Andreasson, D. Arnlund, S. Bajt, T. R. M. Barends, M. Barthelmess, M. J. Bogan, C. Bostedt, J. D. Bozek, R. Coffee, N. Coppola, J. Davidsson, D. P. DePonte, R. B. Doak, T. Ekeberg, V. Elser, S. W. Epp, B. Erk, H. Fleckenstein, L. Foucar, P. Fromme, H. Graafsma, L. Gumprecht, J. Hajdu, C. Y. Hampton, R. Hartmann, A. Hartmann, G. Hauser, H. Hirsemann, P. Holl, M. S. Hunter, L. Johansson, S. Kassemeyer, N. Kimmel, R. A. Kirian, M. Liang, F. R. N. C. Maia, E. Malmerberg, S. Marchesini, A. V. Martin, K. Nass, R. Neutze, C. Reich, D. Rolles, B. Rudek, A. Rudenko, H. Scott, I. Schlichting, J. Schulz, M. M. Seibert, R. L. Shoeman, R. G. Sierra, H. Soltau, J. C. H. Spence, F. Stellato, S. Stern, L. Strueder, J. Ullrich, X. Wang, G. Weidenspointner, U. Weierstall, C. B. Wunderer, and H. N. Chapman, “Self-terminating diffraction gates femtosecond X-ray nanocrystallography measurements,” Nat. Photonics 6, 35 (2012).
http://dx.doi.org/10.1038/nphoton.2011.297
4.
4.L. Lomb, T. R. M. Barends, S. Kassemeyer, A. Aquila, S. W. Epp, B. Erk, L. Foucar, R. Hartmann, B. Rudek, D. Rolles, A. Rudenko, R. L. Shoeman, J. Andreasson, S. Bajt, M. Barthelmess, A. Barty, M. J. Bogan, C. Bostedt, J. D. Bozek, C. Caleman, R. Coffee, N. Coppola, D. P. Deponte, R. B. Doak, T. Ekeberg, H. Fleckenstein, P. Fromme, M. Gebhardt, H. Graafsma, L. Gumprecht, C. Y. Hampton, A. Hartmann, G. Hauser, H. Hirsemann, P. Holl, J. M. Holton, M. S. Hunter, W. Kabsch, N. Kimmel, R. A. Kirian, M. Liang, F. R. N. C. Maia, A. Meinhart, S. Marchesini, A. V. Martin, K. Nass, C. Reich, J. Schulz, M. M. Seibert, R. Sierra, H. Soltau, J. C. H. Spence, J. Steinbrener, F. Stellato, S. Stern, N. Timneanu, X. Wang, G. Weidenspointner, U. Weierstall, T. A. White, C. Wunderer, H. N. Chapman, J. Ullrich, L. Strüder, and I. Schlichting, “Radiation damage in protein serial femtosecond crystallography using an X-ray free-electron laser,” Phys. Rev. B 84, 214111 (2011).
http://dx.doi.org/10.1103/physrevb.84.214111
5.
5.W. Liu, D. Wacker, C. Gati, G. W. Han, D. James, D. Wang, G. Nelson, U. Weierstall, V. Katritch, A. Barty, N. A. Zatsepin, D. Li, M. Messerschmidt, S. Boutet, G. J. Williams, J. E. Koglin, M. M. Seibert, C. Wang, S. T. A. Shah, S. Basu, R. Fromme, C. Kupitz, K. N. Rendek, I. Grotjohann, P. Fromme, R. A. Kirian, K. R. Beyerlein, T. A. White, H. N. Chapman, M. Caffrey, J. C. H. Spence, R. C. Stevens, and V. Cherezov, “Serial femtosecond crystallography of G protein–coupled receptors,” Science 342, 1521 (2013).
http://dx.doi.org/10.1126/science.1244142
6.
6.S. Boutet, L. Lomb, G. J. Williams, T. R. M. Barends, A. Aquila, R. B. Doak, U. Weierstall, D. P. DePonte, J. Steinbrener, R. L. Shoeman, M. Messerschmidt, A. Barty, T. A. White, S. Kassemeyer, R. A. Kirian, M. M. Seibert, P. A. Montanez, C. Kenney, R. Herbst, P. Hart, J. Pines, G. Haller, S. M. Gruner, H. T. Philipp, M. W. Tate, M. Hromalik, L. J. Koerner, N. van Bakel, J. Morse, W. Ghonsalves, D. Arnlund, M. J. Bogan, C. Caleman, R. Fromme, C. Y. Hampton, M. S. Hunter, L. C. Johansson, G. Katona, C. Kupitz, M. Liang, A. V. Martin, K. Nass, L. Redecke, F. Stellato, N. Timneanu, D. Wang, N. A. Zatsepin, D. Schafer, J. Defever, R. Neutze, P. Fromme, J. C. H. Spence, H. N. Chapman, and I. Schlichting, “High-resolution protein structure determination by serial femtosecond crystallography,” Science 337, 362 (2012).
http://dx.doi.org/10.1126/science.1217737
7.
7.L. Redecke, K. Nass, D. P. DePonte, T. A. White, D. Rehders, A. Barty, F. Stellato, M. Liang, T. R. M. Barends, S. Boutet, G. J. Williams, M. Messerschmidt, M. M. Seibert, A. Aquila, D. Arnlund, S. Bajt, T. Barth, M. J. Bogan, C. Caleman, T.-C. Chao, R. B. Doak, H. Fleckenstein, M. Frank, R. Fromme, L. Galli, I. Grotjohann, M. S. Hunter, L. C. Johansson, S. Kassemeyer, G. Katona, R. A. Kirian, R. Koopmann, C. Kupitz, L. Lomb, A. V. Martin, S. Mogk, R. Neutze, R. L. Shoeman, J. Steinbrener, N. Timneanu, D. Wang, U. Weierstall, N. A. Zatsepin, J. C. H. Spence, P. Fromme, I. Schlichting, M. Duszenko, C. Betzel, and H. N. Chapman, “Natively inhibited trypanosoma brucei cathepsin B structure determined by using an X-ray laser,” Science 339, 227 (2013).
http://dx.doi.org/10.1126/science.1229663
8.
8.M. Suga, F. Akita, K. Hirata, G. Ueno, H. Murakami, Y. Nakajima, T. Shimizu, K. Yamashita, M. Yamamoto, H. Ago, and J.-R. Shen, “Native structure of photosystem II at 1.95 Å resolution viewed by femtosecond X-ray pulses,” Nature 517, 99 (2014).
http://dx.doi.org/10.1038/nature13991
9.
9.T. R. M. Barends, L. Foucar, S. Botha, R. B. Doak, R. L. Shoeman, K. Nass, J. E. Koglin, G. J. Williams, S. Boutet, M. Messerschmidt, and I. Schlichting, “De novo protein crystal structure determination from X-ray free-electron laser data,” Nature 505, 244 (2014).
http://dx.doi.org/10.1038/nature12773
10.
10.L. C. Johansson, D. Arnlund, G. Katona, T. A. White, A. Barty, D. P. DePonte, R. L. Shoeman, C. Wickstrand, A. Sharma, G. J. Williams, A. Aquila, M. J. Bogan, C. Caleman, J. Davidsson, R. B. Doak, M. Frank, R. Fromme, L. Galli, I. Grotjohann, M. S. Hunter, S. Kassemeyer, R. A. Kirian, C. Kupitz, M. Liang, L. Lomb, E. Malmerberg, A. V. Martin, M. Messerschmidt, K. Nass, L. Redecke, M. M. Seibert, J. Sjöhamn, J. Steinbrener, F. Stellato, D. Wang, W. Y. Wahlgren, U. Weierstall, S. Westenhoff, N. A. Zatsepin, S. Boutet, J. C. H. Spence, I. Schlichting, H. N. Chapman, P. Fromme, and R. Neutze, “Structure of a photosynthetic reaction centre determined by serial femtosecond crystallography,” Nat. Commun. 4, 2911 (2013).
http://dx.doi.org/10.1038/ncomms3911
11.
11.A. Aquila, M. S. Hunter, R. B. Doak, R. A. Kirian, P. Fromme, T. A. White, J. Andreasson, D. Arnlund, S. Bajt, T. R. M. Barends, M. Barthelmess, M. J. Bogan, C. Bostedt, H. Bottin, J. D. Bozek, C. Caleman, N. Coppola, J. Davidsson, D. P. DePonte, V. Elser, S. W. Epp, B. Erk, H. Fleckenstein, L. Foucar, M. Frank, R. Fromme, H. Graafsma, I. Grotjohann, L. Gumprecht, J. Hajdu, C. Y. Hampton, A. Hartmann, R. Hartmann, S. Hau-Riege, G. Hauser, H. Hirsemann, P. Holl, J. M. Holton, A. H. Mke, L. Johansson, N. Kimmel, S. Kassemeyer, F. Krasniqi, K.-U. K. Hnel, M. Liang, L. Lomb, E. Malmerberg, S. Marchesini, A. V. Martin, F. R. N. C. Maia, M. Messerschmidt, K. Nass, C. Reich, R. Neutze, D. Rolles, B. Rudek, A. Rudenko, I. Schlichting, C. Schmidt, K. E. Schmidt, J. Schulz, M. M. Seibert, R. L. Shoeman, R. Sierra, H. Soltau, D. Starodub, F. Stellato, S. Stern, L. Struder, N. Timneanu, J. Ullrich, X. Wang, G. J. Williams, G. Weidenspointner, U. Weierstall, C. Wunderer, A. Barty, J. C. H. Spence, and H. N. Chapman, “Time-resolved protein nanocrystallography using an X-ray free-electron laser,” Opt. Express 20, 2706 (2012).
http://dx.doi.org/10.1364/oe.20.002706
12.
12.D. Arnlund, L. C. Johansson, C. Wickstrand, A. Barty, G. J. Williams, E. Malmerberg, J. Davidsson, D. Milathianaki, D. P. DePonte, R. L. Shoeman, D. Wang, D. James, G. Katona, S. Westenhoff, T. A. White, A. Aquila, S. Bari, P. Berntsen, M. Bogan, T. B. van driel, R. B. Doak, K. S. Kjær, M. Frank, R. Fromme, I. Grotjohann, R. Henning, M. S. Hunter, R. A. Kirian, I. Kosheleva, C. Kupitz, M. Liang, A. V. Martin, M. M. Nielsen, M. Messerschmidt, M. M. Seibert, J. Sjöhamn, F. Stellato, U. Weierstall, N. A. Zatsepin, J. C. H. Spence, P. Fromme, I. Schlichting, S. Boutet, G. Groenhof, H. N. Chapman, and R. Neutze, “Visualizing a protein quake with time-resolved X-ray scattering at a free-electron laser,” Nat. Methods 11, 923 (2014).
http://dx.doi.org/10.1038/nmeth.3067
13.
13.C. Kupitz, S. Basu, I. Grotjohann, R. Fromme, N. A. Zatsepin, K. N. Rendek, M. S. Hunter, R. L. Shoeman, T. A. White, D. Wang, D. James, J.-H. Yang, D. E. Cobb, B. Reeder, R. G. Sierra, H. Liu, A. Barty, A. L. Aquila, D. Deponte, R. A. Kirian, S. Bari, J. J. Bergkamp, K. R. Beyerlein, M. J. Bogan, C. Caleman, T.-C. Chao, C. E. Conrad, K. M. Davis, H. Fleckenstein, L. Galli, S. P. Hau-Riege, S. Kassemeyer, H. Laksmono, M. Liang, L. Lomb, S. Marchesini, A. V. Martin, M. Messerschmidt, D. Milathianaki, K. Nass, A. Ros, S. Roy-Chowdhury, K. Schmidt, M. Seibert, J. Steinbrener, F. Stellato, L. Yan, C. Yoon, T. A. Moore, A. L. Moore, Y. Pushkar, G. J. Williams, S. Boutet, R. B. Doak, U. Weierstall, M. Frank, H. N. Chapman, J. C. H. Spence, and P. Fromme, “Serial time-resolved crystallography of photosystem II using a femtosecond X-ray laser,” Nature 513, 261 (2014).
http://dx.doi.org/10.1038/nature13453
14.
14.J. Kern, R. Alonso-Mori, R. Tran, J. Hattne, R. J. Gildea, N. Echols, C. Glöckner, J. Hellmich, H. Laksmono, R. G. Sierra, B. Lassalle-Kaiser, S. Koroidov, A. Lampe, G. Han, S. Gul, D. Difiore, D. Milathianaki, A. R. Fry, A. Miahnahri, D. W. Schafer, M. Messerschmidt, M. M. Seibert, J. E. Koglin, D. Sokaras, T.-C. Weng, J. Sellberg, M. J. Latimer, R. W. Grosse-Kunstleve, P. H. Zwart, W. E. White, P. Glatzel, P. D. Adams, M. J. Bogan, G. J. Williams, S. Boutet, J. Messinger, A. Zouni, N. K. Sauter, V. K. Yachandra, U. Bergmann, and J. Yano, “Simultaneous femtosecond X-ray spectroscopy and diffraction of photosystem II at room temperature,” Science 340, 491 (2013).
http://dx.doi.org/10.1126/science.1234273
15.
15.J. Tenboer, S. Basu, N. Zatsepin, K. Pande, D. Milathianaki, M. Frank, M. Hunter, S. Boutet, G. J. Williams, J. E. Koglin, D. Oberthuer, M. Heymann, C. Kupitz, C. Conrad, J. Coe, S. Roy-Chowdhury, U. Weierstall, D. James, D. Wang, T. Grant, A. Barty, O. Yefanov, J. Scales, C. Gati, C. Seuring, V. Srajer, R. Henning, P. Schwander, R. Fromme, A. Ourmazd, K. Moffat, J. J. Van Thor, J. C. H. Spence, P. Fromme, H. N. Chapman, and M. Schmidt, “Time-resolved serial crystallography captures high-resolution intermediates of photoactive yellow protein,” Science 346, 1242 (2014).
http://dx.doi.org/10.1126/science.1259357
16.
16.U. Weierstall, “Liquid sample delivery techniques for serial femtosecond crystallography,” Philos. Trans. R. Soc., B 369, 20130337 (2014).
http://dx.doi.org/10.1098/rstb.2013.0337
17.
17.D. P. DePonte, U. Weierstall, K. Schmidt, J. Warner, D. Starodub, J. C. H. Spence, and R. B. Doak, “Gas dynamic virtual nozzle for generation of microscopic droplet streams,” J. Phys. D 41, 195505 (2008).
http://dx.doi.org/10.1088/0022-3727/41/19/195505
18.
18.U. Weierstall, J. C. H. Spence, and R. B. Doak, “Injector for scattering measurements on fully solvated biospecies,” Rev. Sci. Instrum. 83, 035108 (2012).
http://dx.doi.org/10.1063/1.3693040
19.
19.A. J. Acero, C. Ferrera, J. M. Montanero, and A. M. Gañán-Calvo, “Focusing liquid microjets with nozzles,” J. Micromech. Microeng. 22, 065011 (2012).
http://dx.doi.org/10.1088/0960-1317/22/6/065011
20.
20.A. Gañán-Calvo and J. Montanero, “Revision of capillary cone-jet physics: Electrospray and flow focusing,” Phys. Rev. E 79, 066305 (2009).
http://dx.doi.org/10.1103/physreve.79.066305
21.
21.J. M. Montanero, A. M. Gañán-Calvo, A. J. Acero, and E. J. Vega, “Micrometer glass nozzles for flow focusing,” J. Micromech. Microeng. 20, 075035 (2010).
http://dx.doi.org/10.1088/0960-1317/20/7/075035
22.
22.J. M. Montanero, N. Rebollo-Muñoz, M. A. Herrada, and A. M. Gañán-Calvo, “Global stability of the focusing effect of fluid jet flows,” Phys. Rev. E 83, 036309 (2011).
http://dx.doi.org/10.1103/physreve.83.036309
23.
23.E. J. Vega, J. M. Montanero, M. A. Herrada, and A. M. Gañán-Calvo, “Global and local instability of flow focusing: The influence of the geometry,” Phys. Fluids 22, 064105 (2010).
http://dx.doi.org/10.1063/1.3450321
24.
24.A. M. Gañán-Calvo, “Generation of steady liquid microthreads and micron-sized monodisperse sprays in gas streams,” Phys. Rev. Lett. 80, 285 (1998).
http://dx.doi.org/10.1103/physrevlett.80.285
25.
25.M. Trebbin, K. Krüger, D. Deponte, S. V. Roth, H. N. Chapman, and S. Förster, “Microfluidic liquid jet system with compatibility for atmospheric and high-vacuum conditions,” Lab Chip 14, 1733 (2014).
http://dx.doi.org/10.1039/c3lc51363g
26.
26.J. N. Tilton, in Perry’s Chemical Engineers’ Handbook, 7th ed., edited by D. W. Green and J. O. Maloney (McGraw-Hill, New York, 1999), pp. 6-216-23.
27.
27.Spacer design was inspired by pieces originally manufactured by A. Schleifer of Agilent Technologies, in Collaboration with B. Doak, U. Weierstall, and J. H. C. Spence of Arizona State University (2014).
28.
28.J. M. Montanero, M. A. Herrada, C. Ferrera, E. J. Vega, and A. M. Gañán-Calvo, “On the validity of a universal solution for viscous capillary jets,” Phys. Fluids 23, 122103 (2011).
http://dx.doi.org/10.1063/1.3670007
29.
29.T. Si, F. Li, X.-Y. Yin, and X.-Z. Yin, “Modes in flow focusing and instability of coaxial liquid–gas jets,” J. Fluid Mech. 629, 1 (2009).
http://dx.doi.org/10.1017/s0022112009006211
30.
30.S. Boutet and G. J. Williams, “The coherent X-ray imaging (CXI) instrument at the linac coherent light source (LCLS),” New J. Phys. 12, 035024 (2010).
http://dx.doi.org/10.1088/1367-2630/12/3/035024
31.
31.F. Stellato, D. Oberthür, M. Liang, R. Bean, C. Gati, O. Yefanov, A. Barty, A. Burkhardt, P. Fischer, L. Galli, R. A. Kirian, J. Meyer, S. Panneerselvam, C. H. Yoon, F. Chervinskii, E. Speller, T. A. White, C. Betzel, A. Meents, and H. N. Chapman, “Room-temperature macromolecular serial crystallography using synchrotron radiation,” IUCrJ 1, 204 (2014).
http://dx.doi.org/10.1107/s2052252514010070
32.
32.U. Weierstall, D. James, C. Wang, T. A. White, D. Wang, W. Liu, J. C. H. Spence, R. Bruce Doak, G. Nelson, P. Fromme, R. Fromme, I. Grotjohann, C. Kupitz, N. A. Zatsepin, H. Liu, S. Basu, D. Wacker, G. Won Han, V. Katritch, S. Boutet, M. Messerschmidt, G. J. Williams, J. E. Koglin, M. Marvin seibert, M. Klinker, C. Gati, R. L. Shoeman, A. Barty, H. N. Chapman, R. A. Kirian, K. R. Beyerlein, R. C. Stevens, D. Li, S. T. A. Shah, N. Howe, M. Caffrey, and V. Cherezov, “Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography,” Nat. Commun. 5, 3309 (2014).
http://dx.doi.org/10.1038/ncomms4309
http://aip.metastore.ingenta.com/content/aip/journal/rsi/86/12/10.1063/1.4936843
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/content/aip/journal/rsi/86/12/10.1063/1.4936843
2015-12-08
2016-12-05

Abstract

Serial femtosecond crystallography (SFX) using X-ray Free-Electron Lasers (XFELs) allows for room temperature protein structure determination without evidence of conventional radiation damage. In this method, a liquid suspension of protein microcrystals can be delivered to the X-ray beam in vacuum as a micro-jet, which replenishes the crystals at a rate that exceeds the current XFEL pulse repetition rate. Gas dynamic virtual nozzles produce the required micrometer-sized streams by the focusing action of a coaxial sheath gas and have been shown to be effective for SFX experiments. Here, we describe the design and characterization of such nozzles assembled from ceramic micro-injection molded outer gas-focusing capillaries. Trends of the emitted jet diameter and jet length as a function of supplied liquid and gas flow rates are measured by a fast imaging system. The observed trends are explained by derived relationships considering choked gas flow and liquidflow conservation. Finally, the performance of these nozzles in a SFX experiment is presented, including an analysis of the observed background.

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