Skip to main content

News about Scitation

In December 2016 Scitation will launch with a new design, enhanced navigation and a much improved user experience.

To ensure a smooth transition, from today, we are temporarily stopping new account registration and single article purchases. If you already have an account you can continue to use the site as normal.

For help or more information please visit our FAQs.

banner image
No data available.
Please log in to see this content.
You have no subscription access to this content.
No metrics data to plot.
The attempt to load metrics for this article has failed.
The attempt to plot a graph for these metrics has failed.
The full text of this article is not currently available.
/content/aip/journal/rsi/86/6/10.1063/1.4922068
1.
1.P.-A. Auroux, Y. Koc, A. DeMello, A. Manz, and P. J. R. Day, Lab Chip 4, 534 (2004).
http://dx.doi.org/10.1039/b408850f
2.
2.R. K. Neely, J. Deen, and J. Hofkens, Biopolymers 95, 298 (2011).
http://dx.doi.org/10.1002/bip.21579
3.
3.A. Bensimon, A. Simon, A. Chiffaudel, V. Croquette, F. Heslot, and D. Bensimon, Science 265, 2096 (1994).
http://dx.doi.org/10.1126/science.7522347
4.
4.M. Rief, H. Clausen-Schaumann, and H. Gaub, Nat. Struct. Biol. 6, 346 (1999).
http://dx.doi.org/10.1038/7582
5.
5.S. B. Smith, L. Finzi, and C. Bustamante, Science 258, 1122 (1992).
http://dx.doi.org/10.1126/science.1439819
6.
6.S. Smith, Y. Cui, and C. Bustamante, Science 271, 795 (1996).
http://dx.doi.org/10.1126/science.271.5250.795
7.
7.B. Gibb, T. D. Silverstein, I. J. Finkelstein, and E. C. Greene, Anal. Chem. 84, 7607 (2012).
http://dx.doi.org/10.1021/ac302117z
8.
8.T. Perkins, D. Smith, R. Larson, and S. Chu, Science 268, 83 (1995).
http://dx.doi.org/10.1126/science.7701345
9.
9.R. Marie and A. Kristensen, J. Biophotonics 5, 673 (2012).
http://dx.doi.org/10.1002/jbio.201200050
10.
10.J. O. Tegenfeldt, C. Prinz, H. Cao, S. Chou, W. W. Reisner, R. Riehn, Y. M. Wang, E. C. Cox, J. C. Sturm, P. Silberzan, and R. H. Austin, Proc. Natl. Acad. Sci. U. S. A. 101, 10979 (2004).
http://dx.doi.org/10.1073/pnas.0403849101
11.
11.F. Persson, P. Utko, W. Reisner, N. B. Larsen, and A. Kristensen, Nano Lett. 9, 1382 (2009).
http://dx.doi.org/10.1021/nl803030e
12.
12.J.-W. Yeh, A. Taoni, Y.-L. Chen, and C.-F. Chou, Nano Lett. 12, 1597 (2012).
http://dx.doi.org/10.1021/nl2045292
13.
13.J. W. Larson, G. R. Yantz, Q. Zhong, R. Charnas, C. M. D’Antoni, M. V. Gallo, K. A. Gillis, L. A. Neely, K. M. Phillips, G. G. Wong, S. R. Gullans, and R. Gilmanshin, Lab Chip 6, 1187 (2006).
http://dx.doi.org/10.1039/b602845d
14.
14.T. Perkins, D. Smith, and S. Chu, Science 276, 2016 (1997).
http://dx.doi.org/10.1126/science.276.5321.2016
15.
15.H. P. Chou, C. Spence, A. Scherer, and S. Quake, Proc. Natl. Acad. Sci. U. S. A. 96, 11 (1999).
http://dx.doi.org/10.1073/pnas.96.1.11
16.
16.B. R. Cipriany, P. J. Murphy, J. A. Hagarman, A. Cerf, D. Latulippe, S. L. Levy, J. J. Benítez, C. P. Tan, J. Topolancik, P. D. Soloway, and H. G. Craighead, Proc. Natl. Acad. Sci. U. S. A. 109, 8477 (2012).
http://dx.doi.org/10.1073/pnas.1117549109
17.
17.A. R. Hastie, L. Dong, A. Smith, J. Finklestein, E. T. Lam, N. Huo, H. Cao, P. Y. Kwok, K. R. Deal, J. Dvorak, M. C. Luo, Y. Gu, and M. Xiao, PLoS One 8 (2013).
http://dx.doi.org/10.1371/journal.pone.0055864
18.
18.E. T. Lam, A. Hastie, C. Lin, D. Ehrlich, S. K. Das, M. D. Austin, P. Deshpande, H. Cao, N. Nagarajan, M. Xiao, and P.-Y. Kwok, Nat. Biotechnol. 30, 771 (2012).
http://dx.doi.org/10.1038/nbt.2303
19.
19.R. Marie, J. N. Pedersen, D. L. V. Bauer, K. H. Rasmussen, M. Yusuf, E. Volpi, H. Flyvbjerg, A. Kristensen, and K. U. Mir, Proc. Natl. Acad. Sci. U. S. A. 110, 4893 (2013).
http://dx.doi.org/10.1073/pnas.1214570110
20.
20.S. Tanzi, P. F. Østergaard, M. Matteucci, T. L. Christiansen, J. Cech, R. Marie, and R. Taboryski, J. Micromech. Microeng. 22, 115008 (2012).
http://dx.doi.org/10.1088/0960-1317/22/11/115008
21.
21.See supplementary material at http://dx.doi.org/10.1063/1.4922068 for a time-lapse of a DNA molecule selection and insertion.[Supplementary Material]
22.
22.M. A. Tycon, C. F. Dial, K. Faison, W. Melvin, and C. J. Fecko, Anal. Biochem. 426, 13 (2012).
http://dx.doi.org/10.1016/j.ab.2012.03.021
23.
23.B. K. P. Horn and B. G. Schunck, Artif. Intell. 17, 185 (1981).
http://dx.doi.org/10.1016/0004-3702(81)90024-2
24.
24.M. Tanyeri and C. M. Schroeder, Nano Lett. 13, 2357 (2013).
http://dx.doi.org/10.1021/nl4008437
25.
25.S. Pertuz, D. Puig, and M. A. Garcia, Pattern Recognit. 46, 1415 (2013).
http://dx.doi.org/10.1016/j.patcog.2012.11.011
http://aip.metastore.ingenta.com/content/aip/journal/rsi/86/6/10.1063/1.4922068
Loading
/content/aip/journal/rsi/86/6/10.1063/1.4922068
Loading

Data & Media loading...

Loading

Article metrics loading...

/content/aip/journal/rsi/86/6/10.1063/1.4922068
2015-06-09
2016-12-09

Abstract

We automate the manipulation of genomic-length DNA in a nanofluidic device based on real-time analysis of fluorescence images. In our protocol, individual molecules are picked from a microchannel and stretched with pN forces using pressure driven flows. The millimeter-long DNA fragments free flowing in micro- and nanofluidics emit low fluorescence and change shape, thus challenging the image analysis for machine vision. We demonstrate a set of image processing steps that increase the intrinsically low signal-to-noise ratio associated with single-molecule fluorescence microscopy. Furthermore, we demonstrate how to estimate the length of molecules by continuous real-time image stitching and how to increase the effective resolution of a pressure controller by pulse width modulation. The sequence of image-processing steps addresses the challenges of genomic-length DNA visualization; however, they should also be general to other applications of fluorescence-based microfluidics.

Loading

Full text loading...

/deliver/fulltext/aip/journal/rsi/86/6/1.4922068.html;jsessionid=lQr2WfomNDGe2Gf6kDBtS90v.x-aip-live-02?itemId=/content/aip/journal/rsi/86/6/10.1063/1.4922068&mimeType=html&fmt=ahah&containerItemId=content/aip/journal/rsi
true
true

Access Key

  • FFree Content
  • OAOpen Access Content
  • SSubscribed Content
  • TFree Trial Content
752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
/content/realmedia?fmt=ahah&adPositionList=
&advertTargetUrl=//oascentral.aip.org/RealMedia/ads/&sitePageValue=rsi.aip.org/86/6/10.1063/1.4922068&pageURL=http://scitation.aip.org/content/aip/journal/rsi/86/6/10.1063/1.4922068'
Right1,Right2,Right3,