Laser desorption mass spectrometry for biomolecule detection and its applications
- Conference date: 8-12 October 2000
- Location: Knoxville, Tennessee (USA)
During the past few years, we developed and used laser desorption mass spectrometry for biomolecule detections. Matrix-assisted laser desorption/ionization (MALDI) was successfully used to detect DNA fragments with the size larger than 3000 base pairs. It was also successfully used to sequence DNA with both enzymatic and chemical degradation methods to produce DNA ladders. We also developed MALDI with fragmentation for direct DNA sequencing for short DNA probes. Since laser desorption mass spectrometry for DNA detection has the advantages of fast speed and no need of labeling, it has a great potential for molecular diagnosis for disease and person identification by DNA fingerprinting. We applied laser desorption mass spectrometry to succeed in the diagnosis of cystic fibrosis and several other nerve degenerative diseases such as Huntington’s disease. We also succeeded in demonstrating DNA typing for forensic applications.
- Mass spectrometry
- Matrix assisted laser desorption ionization
- Degenerative diseases
- Forensic science
- Laser applications
- Physics demonstrations
- Sequence analysis
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