Membrane Boundary Extraction Using a Circular Shortest Path Technique
- Conference date: 17–19 December 2007
- Location: Gold Coast, Queensland (Australia)
Membrane proteins represent over 50% of known drug targets. Accordingly, several widely used assays in the High Content Analysis area rely on quantitative measures of the translocation of proteins between intracellular organelles and the cell surface. In order to increase the sensitivity of these assays, one needs to measure the signal specifically along the membrane, requiring a precise segmentation of this compartment. Doing this manually is a very time‐consuming practice, limited to an academic setting. Manual tracing of the membrane compartment also confronts us with issues of objectivity and reproducibility. In this paper, we present an approach based on a circular shortest path technique that enables us to segment the membrane compartment accurately and rapidly. This feature is illustrated using cells expressing epitope‐tagged membrane proteins.
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