- Conference date: 6–7 November 2009
- Location: Firenze (Italy)
Originally developed as a tumor therapy, now photodynamic therapy (PDT) may become a useful tool for treatment of cardiovascular diseases. Different cell types are involved in this vascular pathology, and these cells possess different susceptibility to PDT. In this study we screened the effects of two new photosensitizers (PtS and ALA) on human vascular cells. Human macrophages (Mph), aorta endothelial (HAEC) and smooth muscle (SMC) cells were obtained and cultured as described elsewhere. PtS was added to culture medium 24 h before PDT. ALA was added in concentration in serum‐free culture medium. Then cells were washed carefully and illuminated with 692‐nm (PtS) or 633‐nm (ALA) light. Cellular viability was measured with MTT‐test. Except the case of use 5–10 mM ALA, either photosensitizer accumulation alone or laser illumination alone did not affect cells. Illumination of PtS or ALA–loaded cells impaired cellular viability in dose‐dependent manner. for different vascular cells with PtS were as follows: HAEC ‐1 SMC Mph HAEC and some Mph were unsusceptible to ALA‐PDT. SMC LD90 with ALA was Effects of ALA‐PDT depended on protoporphyrin IX (PpIX) formation in cells. HAEC didn’t accumulate PpIX and were non‐sensitive to ALA‐PDT. PpIX formation in Mph changed individually according to donor. Illumination of ALA‐loaded Mph with low PpIX formation did not affect cells. However for Mph with high PpIX formation comprised All cell types were more susceptible to PtS‐PDT compared to ALA‐PDT. Among tested photosensitizers PtS was the most effective one. HAEC were the most susceptible to PtS‐PDT.
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