- Conference date: 26–30 December 2010
- Location: Manipal, (India)
Atomic Force Microscopy is a powerful tool in studying bacterial systems too. The turgor pressure studies on well known systems like E‐coli and Staphylococcus revealed a fascinating fact that the numbers are in tens of atmosphere depending upon the microbial activity. Hence there is no way that one can destroy them by physical means. This is due to the robust nature of the cell wall. Understanding the cell wall structure requires an estimate of spring constant of the cell wall membrane and its variation upon activity. Here we present an experimental estimate of the spring constant of the cell wall using force curve measurements on bacteria using an AFM tip. This has a bearing on measuring turgor pressure of bacterium.
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