- Conference date: 1–2 February 2013
- Location: Bikaner, Rajasthan, India
The biological silver nanoparticle was synthesized extracellularly by using a fungi Aspergillus foetidus. The live cell filtrate of fungi has been used as reducing agent in the process of nanoparticles synthesis. In 50 ml cell filtrate a volume of stock solution was added to make finally the concentration as 1 mM of and allowed to shake in an incubator for several hrs in dark. The changed color was considered as the primary indication of nanoparticles formation and studies of UV-VIS, DLS, FTIR, AFM, TEM, EDS, Zeta pot. and nitrate reductase assay confirmed the same. It was indicated that stable & 20-40 nm roughly spherical shaped silver nanoparticles was formed. To standardize the nanoparticles biosynthesis different physical parameters like Substrate cone. (0-8 mM), PH-(5-12), Temp.-(5-50°C), incubation time (0-120) hrs and salinity (0.1-1.0 %) were investigated and it was observed that 4 mM conc., PH-9, Temp. −30°C, incubation time 72h and 0.2 % salinity were found to be optimum for the synthesis & stability of the silver nanoparticles.
- Atomic force microscopy
- Biophysical techniques
- Fourier transform infrared spectroscopy
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