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Ultrasonic differentiation of normal versus malignant breast epithelial cells in monolayer cultures
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1.
1.T. E. Doyle, K. H. Warnick, and B. L. Carruth, “Histology-based simulations for the ultrasonic detection of microscopic cancer in vivo,” J. Acoust. Soc. Am. 122, EL210EL216 (2007).
http://dx.doi.org/10.1121/1.2800894
2.
2.T. E. Doyle, A. T. Tew, K. H. Warnick, and B. L. Carruth, “Simulation of elastic wave scattering in cells and tissues at the microscopic level,” J. Acoust. Soc. Am. 125, 17511767 (2009).
http://dx.doi.org/10.1121/1.3075569
3.
3.M. L. Oelze and J. F. Zachary, “Examination of cancer in mouse models using high-frequency quantitative ultrasound,” Ultrasound Med. Biol. 32, 16391648 (2006).
http://dx.doi.org/10.1016/j.ultrasmedbio.2006.05.006
4.
4.R. E. Baddour, M. D. Sherar, J. W. Hunt, G. J. Czarnota, and M. C. Kolios, “High-frequency ultrasound scattering from microspheres and single cells,” J. Acoust. Soc. Am. 117, 934943 (2005).
http://dx.doi.org/10.1121/1.1830668
5.
5.R. E. Baddour and M. C. Kolios, “The fluid and elastic nature of nucleated cells: Implications from the cellular backscatter response,” J. Acoust. Soc. Am. 121, EL16EL22 (2007).
http://dx.doi.org/10.1121/1.2401224
6.
6.L. R. Taggart, R. E. Baddour, A. Giles, G. J. Czarnota, and M. C. Kolios, “Ultrasonic characterization of whole cells and isolated nuclei,” Ultrasound Med. Biol. 33, 389401 (2007).
http://dx.doi.org/10.1016/j.ultrasmedbio.2006.07.037
7.
7.S. Brand, B. Solanki, D. B. Foster, G. J. Czarnota, and M. C. Kolios, “Monitoring of cell death in epithelial cells using high frequency ultrasound spectroscopy,” Ultrasound Med. Biol. 35, 482493 (2009).
http://dx.doi.org/10.1016/j.ultrasmedbio.2008.09.014
8.
8.G. J. Czarnota, M. C. Kolios, J. Abraham, M. Portnoy, F. P. Ottensmeyer, J. W. Hunt, and M. D. Sherar, “Ultrasound imaging of apoptosis: High-resolution non-invasive monitoring of programmed cell death in vitro, in situ, and in vivo,” Br. J. Cancer 81, 520527 (1999).
http://dx.doi.org/10.1038/sj.bjc.6690724
9.
9.R. Banihashemi, R. Vlad, B. Debeljevic, A. Giles, M. C. Kolios, and G. J. Czarnota, “Ultrasound imaging of apoptosis in tumor response: Novel preclinical monitoring of photodynamic therapy effects,” Cancer Res. 68, 85908596 (2008).
http://dx.doi.org/10.1158/0008-5472.CAN-08-0006
10.
10.R. M. Vlad, M. C. Kolios, J. L. Moseley, G. J. Czarnota, and K. K. Brock, “Evaluating the extent of cell death in 3D high frequency ultrasound by registration with whole-mount tumor histopathology,” Med. Phys. 37, 42884297 (2010).
http://dx.doi.org/10.1118/1.3459020
11.
11.I. Bruno, R. E. Kumon, B. Heartwell, E. Maeva, and R. Gr. Maev, “Ex vivo breast tissue imaging and characterization using acoustic microscopy,” in Acoustical Imaging, edited by M. P. André (Springer, Dordrecht, 2007), Vol. 28, pp. 279287.
http://dx.doi.org/10.1007/1-4020-5721-0_29
12.
12.H. D. Soule, T. M. Maloney, S. R. Wolman, W. D. Peterson, Jr., R. Brenz, C. M. McGrath, J. Russo, R. J. Pauley, R. F. Jones, and S. C. Brooks, “Isolation and characterization of a spontaneously immortalized human breast epithelial cell line, MCF-10,” Cancer Res. 50, 60756086 (1990).
13.
13.R. Cailleau, M. Olivé, and Q. V. J. Cruciger, “Long-term human breast carcinoma cell lines of metastatic origin: Preliminary characterization,” In Vitro 14, 911915 (1978).
http://dx.doi.org/10.1007/BF02616120
14.
14.G. C. Gaunaurd and H. Huang, “Acoustic scattering by a spherical body near a plane boundary,” J. Acoust. Soc. Am. 96, 25262536 (1994).
http://dx.doi.org/10.1121/1.410126
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/content/asa/journal/jasa/128/5/10.1121/1.3499699
2010-10-19
2014-12-22

Abstract

Normal and malignant mammary epithelial cells were studied using laboratory measurements, wavelet analysis, and numerical simulations of monolayercell cultures to determine whether microscopic breast cancer can be detected in vitro with high-frequency ultrasound. Pulse-echo waveforms were acquired by immersing a broadband, unfocused 50-MHz transducer in the growth media of cell culture well plates and collecting the first reflection from the well bottoms. The simulations included a multilayer pulse-reflection model and a model of two-dimensional arrays of spherical cells and nuclei. The results show that normal and malignant cells produce time-domain signals and spectral features that are significantly different.

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Scitation: Ultrasonic differentiation of normal versus malignant breast epithelial cells in monolayer cultures
http://aip.metastore.ingenta.com/content/asa/journal/jasa/128/5/10.1121/1.3499699
10.1121/1.3499699
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