1887
banner image
No data available.
Please log in to see this content.
You have no subscription access to this content.
No metrics data to plot.
The attempt to load metrics for this article has failed.
The attempt to plot a graph for these metrics has failed.
oa
Ultrasonic differentiation of normal versus malignant breast epithelial cells in monolayer cultures
Rent:
Rent this article for
Access full text Article
/content/asa/journal/jasa/128/5/10.1121/1.3499699
1.
1.T. E. Doyle, K. H. Warnick, and B. L. Carruth, “Histology-based simulations for the ultrasonic detection of microscopic cancer in vivo,” J. Acoust. Soc. Am. 122, EL210EL216 (2007).
http://dx.doi.org/10.1121/1.2800894
2.
2.T. E. Doyle, A. T. Tew, K. H. Warnick, and B. L. Carruth, “Simulation of elastic wave scattering in cells and tissues at the microscopic level,” J. Acoust. Soc. Am. 125, 17511767 (2009).
http://dx.doi.org/10.1121/1.3075569
3.
3.M. L. Oelze and J. F. Zachary, “Examination of cancer in mouse models using high-frequency quantitative ultrasound,” Ultrasound Med. Biol. 32, 16391648 (2006).
http://dx.doi.org/10.1016/j.ultrasmedbio.2006.05.006
4.
4.R. E. Baddour, M. D. Sherar, J. W. Hunt, G. J. Czarnota, and M. C. Kolios, “High-frequency ultrasound scattering from microspheres and single cells,” J. Acoust. Soc. Am. 117, 934943 (2005).
http://dx.doi.org/10.1121/1.1830668
5.
5.R. E. Baddour and M. C. Kolios, “The fluid and elastic nature of nucleated cells: Implications from the cellular backscatter response,” J. Acoust. Soc. Am. 121, EL16EL22 (2007).
http://dx.doi.org/10.1121/1.2401224
6.
6.L. R. Taggart, R. E. Baddour, A. Giles, G. J. Czarnota, and M. C. Kolios, “Ultrasonic characterization of whole cells and isolated nuclei,” Ultrasound Med. Biol. 33, 389401 (2007).
http://dx.doi.org/10.1016/j.ultrasmedbio.2006.07.037
7.
7.S. Brand, B. Solanki, D. B. Foster, G. J. Czarnota, and M. C. Kolios, “Monitoring of cell death in epithelial cells using high frequency ultrasound spectroscopy,” Ultrasound Med. Biol. 35, 482493 (2009).
http://dx.doi.org/10.1016/j.ultrasmedbio.2008.09.014
8.
8.G. J. Czarnota, M. C. Kolios, J. Abraham, M. Portnoy, F. P. Ottensmeyer, J. W. Hunt, and M. D. Sherar, “Ultrasound imaging of apoptosis: High-resolution non-invasive monitoring of programmed cell death in vitro, in situ, and in vivo,” Br. J. Cancer 81, 520527 (1999).
http://dx.doi.org/10.1038/sj.bjc.6690724
9.
9.R. Banihashemi, R. Vlad, B. Debeljevic, A. Giles, M. C. Kolios, and G. J. Czarnota, “Ultrasound imaging of apoptosis in tumor response: Novel preclinical monitoring of photodynamic therapy effects,” Cancer Res. 68, 85908596 (2008).
http://dx.doi.org/10.1158/0008-5472.CAN-08-0006
10.
10.R. M. Vlad, M. C. Kolios, J. L. Moseley, G. J. Czarnota, and K. K. Brock, “Evaluating the extent of cell death in 3D high frequency ultrasound by registration with whole-mount tumor histopathology,” Med. Phys. 37, 42884297 (2010).
http://dx.doi.org/10.1118/1.3459020
11.
11.I. Bruno, R. E. Kumon, B. Heartwell, E. Maeva, and R. Gr. Maev, “Ex vivo breast tissue imaging and characterization using acoustic microscopy,” in Acoustical Imaging, edited by M. P. André (Springer, Dordrecht, 2007), Vol. 28, pp. 279287.
http://dx.doi.org/10.1007/1-4020-5721-0_29
12.
12.H. D. Soule, T. M. Maloney, S. R. Wolman, W. D. Peterson, Jr., R. Brenz, C. M. McGrath, J. Russo, R. J. Pauley, R. F. Jones, and S. C. Brooks, “Isolation and characterization of a spontaneously immortalized human breast epithelial cell line, MCF-10,” Cancer Res. 50, 60756086 (1990).
13.
13.R. Cailleau, M. Olivé, and Q. V. J. Cruciger, “Long-term human breast carcinoma cell lines of metastatic origin: Preliminary characterization,” In Vitro 14, 911915 (1978).
http://dx.doi.org/10.1007/BF02616120
14.
14.G. C. Gaunaurd and H. Huang, “Acoustic scattering by a spherical body near a plane boundary,” J. Acoust. Soc. Am. 96, 25262536 (1994).
http://dx.doi.org/10.1121/1.410126
http://aip.metastore.ingenta.com/content/asa/journal/jasa/128/5/10.1121/1.3499699
Loading
View: Figures

Figures

Image of FIG. 1.

Click to view

FIG. 1.

Phase-contrast micrographs of (a) normal MCF-10A and (b) malignant MDA-MB-468 breast epithelial cells grown to confluence, showing the larger cell and nucleus sizes of the malignant versus normal cells.

Image of FIG. 2.

Click to view

FIG. 2.

(a) Cell growth curves for normal and malignant breast epithelial cells in monolayer cultures grown in vitro. (b) Comparison of ultrasonic waveforms from Day 0 (dashed) and Day 9 (solid) of a monolayer culture of normal cells. Cell layer reflection occurs at approximately 7200 ns on Day 9 waveform (arrow). Waveforms are offset in time for clarity.

Image of FIG. 3.

Click to view

FIG. 3.

(a) Amplitudes of the first wave reflections from the normal cell monolayers, showing the increase in amplitude of the waveform valley with time. (b) Amplitudes of the first wave reflections from the malignant cell monolayers, showing the increase in amplitude of the waveform peak with time.

Image of FIG. 4.

Click to view

FIG. 4.

Wavelet analysis of (a) Day 9 of normal culture well, and (b) Day 9 of malignant culture well. The wavelet analysis highlights the differences in frequency content between the reflections of the normal cell monolayer and the malignant cell monolayer.

Image of FIG. 5.

Click to view

FIG. 5.

(a) Computed ultrasonic backscatter spectra of normal and malignant cell monolayers in aqueous growth media. The peaks for malignant cells display a shift to lower frequencies due to their greater cell and nucleus size. (b) Computed ultrasonic backscatter spectra of normal and malignant cell monolayers in a polystyrene matrix to estimate the effects of the well surface on cell scattering.

Loading

Article metrics loading...

/content/asa/journal/jasa/128/5/10.1121/1.3499699
2010-10-19
2014-04-23

Abstract

Normal and malignant mammary epithelial cells were studied using laboratory measurements, wavelet analysis, and numerical simulations of monolayercell cultures to determine whether microscopic breast cancer can be detected in vitro with high-frequency ultrasound. Pulse-echo waveforms were acquired by immersing a broadband, unfocused 50-MHz transducer in the growth media of cell culture well plates and collecting the first reflection from the well bottoms. The simulations included a multilayer pulse-reflection model and a model of two-dimensional arrays of spherical cells and nuclei. The results show that normal and malignant cells produce time-domain signals and spectral features that are significantly different.

Loading

Full text loading...

/deliver/fulltext/asa/journal/jasa/128/5/1.3499699.html;jsessionid=idec3kha8l5r.x-aip-live-01?itemId=/content/asa/journal/jasa/128/5/10.1121/1.3499699&mimeType=html&fmt=ahah&containerItemId=content/asa/journal/jasa
true
true
This is a required field
Please enter a valid email address
752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Ultrasonic differentiation of normal versus malignant breast epithelial cells in monolayer cultures
http://aip.metastore.ingenta.com/content/asa/journal/jasa/128/5/10.1121/1.3499699
10.1121/1.3499699
SEARCH_EXPAND_ITEM