Index of content:
Volume 130, Issue 6, December 2011
- BIOACOUSTICS 
Model and experimental analysis of oblique incident ultrasound in a tissue layer using doublet mechanics theory130(2011); http://dx.doi.org/10.1121/1.3658471View Description Hide Description
The fundamental framework of doublet mechanics (DM) is used to analyze high-frequency ultrasoundwave propagation in materials with discrete microstructure. Ultrasonicreflection coefficients were measured from a thin layer of tissue embedded between two glass substrates at oblique incidence. Theoretical calculations for the reflection coefficients of a multi-layered system at oblique angles are performed using both DM theory and the classical continuum mechanics theory (CCM). For example, at the frequency of 10 MHz at incident angle 8° in sample with 30 μm thickness, the discrepancy in the magnitude of the reflection coefficient between experimental results and theoretical prediction is 15.8% for DM but 79.0% for CCM; similar results at other frequencies and incident angle in the samples with 30 and 60 μm thickness have also been obtained, which demonstrates that the DM theory can better describe the wave propagation in tissue. The influence of the incident angles and tissue thickness are also discussed in this paper.
Estimating material viscoelastic properties based on surface wave measurements: A comparison of techniques and modeling assumptions130(2011); http://dx.doi.org/10.1121/1.3655883View Description Hide Description
Previous studies of the first author and others have focused on low audible frequency (<1 kHz) shear and surface wave motion in and on a viscoelastic material comprised of or representative of soft biological tissue. A specific case considered has been surface (Rayleigh) wave motion caused by a circular disk located on the surface and oscillating normal to it. Different approaches to identifying the type and coefficients of a viscoelasticmodel of the material based on these measurements have been proposed. One approach has been to optimize coefficients in an assumed viscoelasticmodel type to match measurements of the frequency-dependent Rayleigh wave speed. Another approach has been to optimize coefficients in an assumed viscoelasticmodel type to match the complex-valued frequency response function (FRF) between the excitation location and points at known radial distances from it. In the present article, the relative merits of these approaches are explored theoretically, computationally, and experimentally. It is concluded that matching the complex-valued FRF may provide a better estimate of the viscoelasticmodel type and parameter values; though, as the studies herein show, there are inherent limitations to identifying viscoelastic properties based on surface wavemeasurements.
Ultrasonic backscatter coefficient quantitative estimates from high-concentration Chinese hamster ovary cell pellet biophantoms130(2011); http://dx.doi.org/10.1121/1.3655879View Description Hide Description
Previous work estimated the ultrasonicbackscatter coefficient (BSC) from low-concentration (volume density < 3%) Chinese Hamster Ovary (CHO, 6.7 -μm cell radius) cell pellets. This study extends the work to higher cell concentrations (volume densities: 9.6% to 63%). At low concentration, BSC magnitude is proportional to the cell concentration and BSC frequency dependency is independent of cell concentration. At high cell concentration, BSC magnitude is not proportional to cell concentration and BSC frequency dependency is dependent on cell concentration. This transition occurs when the volume density reaches between 10% and 30%. Under high cell concentration conditions, the BSC magnitude increases slower than proportionally with the number density at low frequencies (ka < 1), as observed by others. However, what is new is that the BSC magnitude can increase either slower or faster than proportionally with number density at high frequencies (ka > 1). The concentric sphere model least squares estimates show a decrease in estimated cell radius with number density, suggesting that the concentric spheres model is becoming less applicable as concentration increases because the estimated cell radius becomes smaller than that measured. The critical volume density, starting from when the model becomes less applicable, is estimated to be between 10% and 30% cell volume density.
130(2011); http://dx.doi.org/10.1121/1.3651815View Description Hide Description
The vocalization behavior of Mongolian gerbils, a model animal of auditory physiology, was examined. A pair of gerbils was placed in a chamber, and their species-specific vocalizations and locomotive behaviors were recorded and analyzed. Two types of calls were predominantly produced: high-frequency upward frequency-modulated (HU-FM) calls and low-frequency multi-harmonic frequency-modulated (LM-FM) calls. Emission rates of HU-FM calls significantly decreased as the distance between the two gerbils increased, and playback of simulated HU-FM calls increased the emission rates. Acoustic analysis of HU-FM calls showed that the calls exhibited a stereotypic spectro-temporal structure including a fixed inter-onset interval (100–175 ms) and that individual differences in the frequency could convey the body size of the callers. The timing of HU-FM calls was highly synchronized with jump movements when an animal vocalized while jumping, suggesting the existence of tight locomotor-vocal coupling. Conversely, LM-FM calls were observed only when the gerbils tactilely contacted with each other while fighting over a food. These results suggest that Mongolian gerbils change the rates of call emissions and call types (e.g., LM-FM or HU-FM calls) in response to changes in visual and possibly tactile and auditory information. The functions of both calls are discussed in terms of their acoustic structures.
130(2011); http://dx.doi.org/10.1121/1.3652898View Description Hide Description
Coronary artery disease (CAD) is the leading cause of death in the United States, being responsible for more than 20% of all deaths in the country. This is in large part due to the difficulty of diagnostic screening for CAD. Phonoangiography seeks to detectCAD via the acoustic signature associated with turbulent flow near an abnormally constricted, or stenosed, region. However, the usefulness of the technique is severely hindered by the low strength of the CAD signal compared to the background noise within the chest. In this work, acoustic finite element analysis (FEA) was performed on physiologically accurate chest geometries to demonstrate the feasibility of an original acoustic source separation methodology for isolating coronary sounds. This approach is based upon pseudoinversion of mixing matrices determined through a combination of experiment and computation. This allows calculation of the sound emitted by the coronary arteries based upon measurements of the acoustic velocity on the chest surface. This work demonstrates the feasibility of such a technique computationally and examines the vulnerability of the proposed approach to measurement errors.