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Creating cellular patterns using genetically engineered, gold- and cell-binding polypeptides
A. Hucknall, A. J. Simnick, R. T. Hill, A. Chilkoti, A. Garcia, M. S. Johannes, R. L. Clark, S. Zauscher, and B. D. Ratner, Biointerphases 4, FA50 (2009).
M. Hnilova, D. Khatayevich, A. Carlson, E. E. Oren, C. Gresswell, S. Zheng, F. Ohuchi, M. Sarikaya, and C. Tamerler, J. Colloid Interface Sci. 365, 97 (2012).
T. Kacar, M. T. Zin, C. So, B. Wilson, H. Ma, N. Gul-Karaguler, A. K.-Y. Jen, M. Sarikaya, and C. Tamerler, Biotechnol. Bioeng. 103, 696 (2009).
W. Hassouneh, T. Christensen, and A. Chilkoti, “ Unit–6.11 Elastin-like polypeptides as a purification tag for recombinant proteins,” Current Protocols in Protein Science / Editorial Board, edited by John E. Coligan et al. (2010).
D. E. Meyer and A. Chilkoti, “ Protein purification by inverse transition cycling,” Protein–Protein Interaction: A Molecular Cloning Manual, edited by Erica Golemis ( Cold Spring Harbor Laboratory, New York, 2002), Chap. 18, p. 329.
M. Rodahl, F. Höök, C. Fredriksson, C. A. Keller, A. Krozer, P. Brzezinski, M. Voinova, and B. Kasemo, Faraday Discuss. 107, 229 (1997).
T. Togashi, N. Yokoo, M. Umetsu, S. Ohara, T. Naka, S. Takami, H. Abe, I. Kumagai, and T. Adschiri, J. Biosci. Bioeng. 111, 140 (2011).
See supplementary material at http://dx.doi.org/10.1116/1.4952452
for details on the setup for controlled washing of cells bound to surfaces, fluorescence intensity analysis of peptide modified substrates, thickness of peptide layers, thermal characterization of RGD-ELP-Cys, and quantitative analysis of cell adhesion behavior on peptide modified surfaces at different temperatures.[Supplementary Material]
cells on material surfaces is an important tool for the study of fundamental cell biology, tissue engineering, and cell-based bioassays. Here, the authors report a simple approach to pattern
cells on gold
silicon substrates with high precision, fidelity, and stability. Cell
patterning is achieved by exploiting adsorbed biopolymer orientation to either enhance (gold regions) or impede (silicon oxide regions) cell adhesion at particular locations on the patterned
surface. Genetic incorporation of gold binding domains enables C-terminal chemisorption of polypeptides onto gold regions with enhanced accessibility of N-terminal cell binding domains. In contrast, the orientation of polypeptides adsorbed on the silicon oxide regions limit the accessibility of the cell binding domains. The dissimilar accessibility of cell binding domains on the gold and silicon oxide regions directs the cell adhesion in a spatially controlled manner in serum-free medium, leading to the formation of well-defined cellular
patterns. The cells are confined within the polypeptide-modified gold regions and are viable for eight weeks, suggesting that bioactive polypeptide modified surfaces are suitable for long-term maintenance of patterned
cells. This study demonstrates an innovative surface-engineering approach for cell
patterning by exploiting distinct ligand accessibility on heterogeneous surfaces.
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