SEM images of two luting agents in contact with dentin. With this technique, differences between the bonding of the cements at the interface cannot be discerned.
FIB lift out method used to produce TEM lamellae from the bulk specimens for (a) Fuji I and (b) Ceramir Crown and Bridge. The images describe the sequence of processing events from, 1) selection of the area of interest at the interface and depositing a protective carbon layer, 2) rough milling of trenches on either side of the protected region of interest, 3) cutting free of the sample and lift out with in situ micromanipulator, and 4) attachment of the lamella to a TEM grid and final ion beam thinning to electron transparency.
STEM HAADF images of the cement–dentin interface at low (a), (c) and higher magnification (b), (d).
(Color online) Electron tomograms of the cementing agents in contact with dentin. Both cements display intimate contact with dentin on the nanometer scale, with the Fuji I cement suggesting an intermediate zone at the interface. The three-dimensional structure can be more fully appreciated in the supplementary videos 3 and 4 (Ref. 16 ).
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