Enzyme adsorption on polymer-based confined bioinspired biosensing surface
Schematic showing the process steps performed on PS-b-PMMA thin films to create nanopores.
Schematic of the conversion of cholesterol into a colored pentaenylic cation upon addition of the Liebermann–Burchard reagent (adapted from Ref. 27).
(Color online) AFM height images illustrating the various processing steps attempted to induce the desired pore nanostructure on PS-b-PMMA thin films: (a) heat treatment only, (b) heat treatment followed by solvent annealing, (c) heat treatment and solvent annealing followed by a 1 h solvent soak, and (d) heat treatment and solvent annealing followed by a 20 h solvent soak.
(Color online) (a) Fluorescence image and (b) large scan size AFM height image showing the location of cholesterol oxidase aggregates.
(Color online) AFM height images taken using (a) 50 × 50, (b) 20 × 20, (c) 5 × 5, and (d) 1 × 1 μm2 scan sizes. In the larger scan size image, boxes are drawn to denote the area taken for the smaller size (higher resolution) image.
(Color online) Examples of the superimposition of the height and phase scan lines of block copolymer surface without and with added ChOx. (a)Height image, (b) phase image, and (c) superimposed scan lines for block copolymer without ChOx. (d) Height image, (e) phase image, and (f) superimposed scan lines for block copolymer with added ChOx. The jumps in the phase image correspond to the presence of cholesterol oxidase on the PS-b-PMMA. The solid and dotted lines correspond to the height and phase scan lines, respectively.
UV–VIS absorbance spectra of cholesterol solutions assayed with the Liebermann–Burchard reagent. The absorbance of the cholesterol solution exposed to ChOx in the solid phase (block copolymer/glass) is less than that of the unreacted cholesterol solution, implying that ChOx catalyzed cholesterol.
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