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Desorption/ionization mass spectrometry on array of silicon microtips
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10.1116/1.1861046
/content/avs/journal/jvstb/23/2/10.1116/1.1861046
http://aip.metastore.ingenta.com/content/avs/journal/jvstb/23/2/10.1116/1.1861046
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Figures

Image of FIG. 1.
FIG. 1.

(a) Schematic view of MALDI TOF MS equipment (Bruker Saxonia), and (b) mass spectrogram of dopamine. Note the background signal generated by the matrix (4-hydroxy--cyanocinnamic acid).

Image of FIG. 2.
FIG. 2.

(a) Principle of desorption/ionization of a biosample for the MALDI method (hydrophobic spot on the target plate), the DIOS method (porous silicon spot on a silicon wafer), and the DIOSD method (porous silicon dioxide spot on a silicon wafer) in comparison with (b) the DIOSTA method.

Image of FIG. 3.
FIG. 3.

Cross-sectional view of the gated silicon tips.

Image of FIG. 4.
FIG. 4.

Step-by-step fabrication process of the array of gated silicon tips.

Image of FIG. 5.
FIG. 5.

Scanning electron microscopy pictures of silicon wafer: (a) after plasma etching of silicon to form tips; (b) after deposition of plasma-enhanced chemical-vapor deposition layer, Cr–Au bilayer and photoresist (AZ); and (c) after opening windows in photoresist layer.

Image of FIG. 6.
FIG. 6.

(a) DIOSTA platform with three spots and (b) a few gated silicon tips not covered with the biosample.

Image of FIG. 7.
FIG. 7.

Mass spectrogram of dopamine 65 pmol per spot: (a) DIOSTA method, spectrogram was obtained for 100 laser-light pulses and (b) DIOSD method, spectrogram was obtained for 165 laser-light pulses. Positive (protonized) molecular ion at a m/z of 154.1 and its fragment at 137.03, as well as signals at m/z’s of 176.0 and 192.0, which are sodium and potassium adducts of dopamine, may be seen.

Image of FIG. 8.
FIG. 8.

Mass spectrogram of LGG tripetide 40 pmol per spot: (a) DIOSTA method, spectrogram was obtained for 15 laser-light pulses; (b) DIOS method; and (c) DIOSD method. Spectrograms (b) and (c) were obtained for 165 laser-light pulses. Positive (protonized) molecular ion at a m/z of 246.2 and signals at m/z’s of 268.2 and 284.2, which are sodium and potassium adducts of tripeptide, may be seen.

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/content/avs/journal/jvstb/23/2/10.1116/1.1861046
2005-04-07
2014-04-21
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Desorption/ionization mass spectrometry on array of silicon microtips
http://aip.metastore.ingenta.com/content/avs/journal/jvstb/23/2/10.1116/1.1861046
10.1116/1.1861046
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