Development of a regeneration-type neural interface: A microtube guide for axon growth of neuronal cells fabricated using focused-ion-beam chemical vapor deposition
Bundle of DLC microtubes attached to the edge of a glass capillary. These had an inner diameter of about .
Bifurcated microtube attached to a glass capillary. The inner diameter of the end of the microtube was about .
Carbon microtube on a porous polycarbonate membrane with pore diameter of . The microtube was about in height and in inner diameter as observed by SEM.
Schematic of the in vitro test using a glass capillary with a bifurcated microtube. The glass capillary and the microtubes were immersed in a culture medium with of NGF added. PC12 cells were cultured in the capillary.
Schematic of the in vitro test using polycarbonate membranes with straight microtubes. The membranes and the microtubes were immersed in a culture medium with of NGF added. PC12 cells were cultured on the membranes.
Axons of PC12 extend on DLC thin film ( fabricated by FIB-CVD) at day 2 (day 0: beginning; day 1: addition of NGF; day 2: optical observation). The sharpness of this image was increased by digital image processing.
Bifurcated microtube in the medium and PC12 cells in a glass capillary observed by optical microscope: (a) bifurcated microtube; (b) differentiated PC12 cells at the tip of the glass capillary. The neurite was observed, but axon extension was not observed.
Axon extension on a microtube attached to a polycarbonate membrane. (a) SEM image of the microtube and the axon. (b) Schematic of SEM image in (a). The axon grew from the upper right of (a) to the microtube, extended along the inner surface of the microtube, and then exited from it.
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