1887
banner image
No data available.
Please log in to see this content.
You have no subscription access to this content.
No metrics data to plot.
The attempt to load metrics for this article has failed.
The attempt to plot a graph for these metrics has failed.
Nanoimprint fabrication of polymer cell substrates with combined microscale and nanoscale topography
Rent:
Rent this article for
USD
10.1116/1.2748792
/content/avs/journal/jvstb/25/4/10.1116/1.2748792
http://aip.metastore.ingenta.com/content/avs/journal/jvstb/25/4/10.1116/1.2748792
View: Figures

Figures

Image of FIG. 1.
FIG. 1.

Schematic of the hot embossing process. In this process a silicon micromachined master is brought into contact with a polymer substrate under heat and force. After the desired embossing time, the mold is cooled and removed leaving replicate features in the polymer substrate.

Image of FIG. 2.
FIG. 2.

Schematic of the second embossing. A nanopatterned nickel master is brought into contact with the micropatterned polymer under lower temperature and higher pressure than the previous embossing process. The mold is then cooled and removed from the substrate leaving nanogrooves on the micromesas.

Image of FIG. 3.
FIG. 3.

Scanning electron micrographs of double embossed substrates. Top image shows nanogrooves on the micromesas running parallel to the microgrooves. Bottom image shows nanogrooves running perpendicular to the microgrooves.

Image of FIG. 4.
FIG. 4.

Alignment results for cells cultured on microgrooves, microgrooves with parallel nanogrooves, and microgrooves with perpendicular nanogrooves. Results show alignment trends for microgroove spacings of 2 and . and (#) denote significant interactions between perpendicular nanogrooves and both microgrooves only and parallel nanogrooves for 2 and , respectively. The significant interaction parameters for 2 and patterns were less than 0.02 and 0.01, respectively. Alignment of cells on nanogrooves and a flat control surface is also shown for reference.

Image of FIG. 5.
FIG. 5.

Immunofluorescence image of cell cytoskeletons on microgrooved surface with nanogrooves running perpendicular. Cell extensions are visible, running along the nanogrooves while cell bodies appear to be aligning to the microgrooves.

Image of FIG. 6.
FIG. 6.

Scanning electron microscope (SEM) image of cell on microgrooves with nanogrooves running perpendicular. Cell extensions are extending out from the cell body and running along the nanogrooves. Extension also spans groove with no nanopattern to mesa with nanopatterning.

Loading

Article metrics loading...

/content/avs/journal/jvstb/25/4/10.1116/1.2748792
2007-06-21
2014-04-19
Loading

Full text loading...

This is a required field
Please enter a valid email address
752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Nanoimprint fabrication of polymer cell substrates with combined microscale and nanoscale topography
http://aip.metastore.ingenta.com/content/avs/journal/jvstb/25/4/10.1116/1.2748792
10.1116/1.2748792
SEARCH_EXPAND_ITEM