(a) Optical image of a device consisting of two reservoirs connected by a random fiber mat, taken at the third process step after the PDMS has been poured over the template and cured. (b) Schematic of a typical fabrication process.
SEM images of nanochannel cross sections. In both images, the glass coverslip is below the interface and the PDMS layer is above the interface. Images (a) and (b) show larger and smaller diameter channels, respectively.
Fluorescence image of randomly aligned channels filling with a solution of the fluorescent dye fluorescein.
Fluorescence image of aligned channels connecting two reservoirs. The reservoir on the left has already filled with dye, and the dye has flowed through the channels and has begun to fill the reservoir on the right.
Fluorescence image indicating the paths of single molecules of DNA through fluidic channels. Several frames from a video of the DNA molecules in the device were superimposed to create this image. Both the exposure time and the temporal gap between each of the DNA images were . The video is available in the supplementary material.
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