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Fabrication of nanoscale bioarrays for the study of cytoskeletal protein binding interactions using nanoimprint lithography
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10.1116/1.3043472
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    Affiliations:
    1 Department of Chemical Engineering, Nanotechnology Center for Mechanics in Regenerative Medicine, Columbia University, New York, New York 10027
    2 Department of Biological Sciences, Nanotechnology Center for Mechanics in Regenerative Medicine, Columbia University, New York, New York 10027
    3 Department of Mechanical Engineering, Nanotechnology Center for Mechanics in Regenerative Medicine, Columbia University, New York, New York 10027
    4 Department of Biological Sciences, Nanotechnology Center for Mechanics in Regenerative Medicine, Columbia University, New York, New York 10027
    5 Department of Mechanical Engineering, Nanotechnology Center for Mechanics in Regenerative Medicine, Columbia University, New York, New York 10027
    6 Department of Biological Sciences, Nanotechnology Center for Mechanics in Regenerative Medicine, Columbia University, New York, New York 10027
    7 Department of Applied Physics and Applied Mathematics, Nanotechnology Center for Mechanics in Regenerative Medicine, Columbia University, New York, New York 10027
    a) Electronic mail: sw2l28@columbia.edu
    J. Vac. Sci. Technol. B 27, 61 (2009); http://dx.doi.org/10.1116/1.3043472
/content/avs/journal/jvstb/27/1/10.1116/1.3043472
http://aip.metastore.ingenta.com/content/avs/journal/jvstb/27/1/10.1116/1.3043472
View: Figures

Figures

Image of FIG. 1.
FIG. 1.

(Color online) Schematic of the nanoarray chip. The chip contains an array of lattices with varying parameter , which defines the distance between protein binding sites, and a constant distance between neighboring pairs (c) of 200 nm. Each lattice has the same total number of binding sites.

Image of FIG. 2.
FIG. 2.

(Color online) Schematic process flow of array fabrication.

Image of FIG. 3.
FIG. 3.

PMMA, imprinted with the template that defines arrays of pairs of 15–20 nm diameter dots.

Image of FIG. 4.
FIG. 4.

Cross section scanning electron microscopy (SEM) image of nanopatterned array before lift-off.

Image of FIG. 5.
FIG. 5.

SEM images of the array of AuPd dots (a) after lift-off and [(b) and (c)] after annealing.

Image of FIG. 6.
FIG. 6.

(Color online) (a) Optical microscope image of “” area in the NIL template, (b) same area on the imprinted glass sample, biofunctionalized with thiolated biotin and fluorescence-tagged avidin.

Image of FIG. 7.
FIG. 7.

(Color online) (a) Optical microscopy of the patterned arrays before lift-off. (b) Cells spreading on the patterned substrate, biofunctionalized with RGD. (The test area consists of features patterned for lithography control purposes and is not measured in cell spreading experiments.)

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/content/avs/journal/jvstb/27/1/10.1116/1.3043472
2009-01-12
2014-04-25
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Fabrication of nanoscale bioarrays for the study of cytoskeletal protein binding interactions using nanoimprint lithography
http://aip.metastore.ingenta.com/content/avs/journal/jvstb/27/1/10.1116/1.3043472
10.1116/1.3043472
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