Fabrication of nanoscale bioarrays for the study of cytoskeletal protein binding interactions using nanoimprint lithography
(Color online) Schematic of the nanoarray chip. The chip contains an array of lattices with varying parameter , which defines the distance between protein binding sites, and a constant distance between neighboring pairs (c) of 200 nm. Each lattice has the same total number of binding sites.
(Color online) Schematic process flow of array fabrication.
PMMA, imprinted with the template that defines arrays of pairs of 15–20 nm diameter dots.
Cross section scanning electron microscopy (SEM) image of nanopatterned array before lift-off.
SEM images of the array of AuPd dots (a) after lift-off and [(b) and (c)] after annealing.
(Color online) (a) Optical microscope image of “” area in the NIL template, (b) same area on the imprinted glass sample, biofunctionalized with thiolated biotin and fluorescence-tagged avidin.
(Color online) (a) Optical microscopy of the patterned arrays before lift-off. (b) Cells spreading on the patterned substrate, biofunctionalized with RGD. (The test area consists of features patterned for lithography control purposes and is not measured in cell spreading experiments.)
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