Development of microfluidic device and system for breast cancer cell fluorescence detection
(Color online) Schematic of top view of the microfluidics device.
(Color online) Schematic of cross section of microfluidics device, and chamber 2 is used for capturing microparticles.
(Color online) Image of a microfluidic device (a) inside the plastic testing holder connected to the syringe pump and the pressure gauge and (b) the device in hand; SEM images of (c) the silicon pillars inside the microfluidics device, and (d) part of meandering U-shaped silicon weirs (for cell capture) while the Pyrex cover is opened.
(Color online) Overlap of two fluorescence images of the top view of the U-shape meandering weirs. One image was taken with the filter set for the blue Hoechst dye for MCF-10A, while the other one was taken with the filter set for the green FITC dye for MCF-7. Both captured cancer cells MCF-7 and control cells MCF-10A are shown as the arrow indicated.
(Color online) Fluorescence signal intensity (from 0 to 4096) against labeling reaction time between the -BSA-FITC and MCF-7 cancer cells on a glass slide and under flow in the Si/glass microfluidic device
Comparison between MCF-7 and MCF-10A and their response against labels.
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