Gold-tipped elastomeric pillars for cellular mechanotransduction
(a) Scanning electron micrograph of the PDMS posts. The diameter of the posts is and the pitch is . The height of the posts is . The stiffness of the post is about AAA. (b) Bright-field micrograph of the spreading of mouse embryonic fibroblast cell on the pillar substrate.
Schematic of the process flow for fabricating arrays of the PDMS posts with functionalized Au on the top surface.
(a) SEM image of holes in the silicon substrate. The diameter of the holes is and the pitch size is . The height of the hole is . (b) SEM image of final Si mold with a thin layer of Au and Ti at the bottom of the holes.
Scanning electron micrograph of Au-tipped PDMS pillars. The tops of the pillars can be selectively chemical functionalized.
Epifluorescence image of the tip of the functionalized pillars with diameter of and center-to-center distance of . The tops of the pillars are coated homogeneously with fluorescently labeled fibronectin and there is no signal from the bottom of the pillars.
Image of the bottom of the pillars using confocal laser scanning microscopy. (a) The fluorescence signal of functionalized pillars decreases as along the length of the pillar, indicating that signal is coming from the top of pillars. (b) Rings of fluorescence signal around the sidewall of pillars in a nonfunctionalized substrate, indicating the complete coating of the sidewall.
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