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Gold-tipped elastomeric pillars for cellular mechanotransduction
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10.1116/1.3259953
    + View Affiliations - Hide Affiliations
    Affiliations:
    1 Department of Mechanical Engineering, Columbia University, New York, New York 10027 and Nanotechnology Center for Mechanics in Regenerative Medicine, Columbia University, New York, New York 10027
    2 Department of Biological Sciences, Columbia University, New York, New York 10027 and Nanotechnology Center for Mechanics in Regenerative Medicine, Columbia University, New York, New York 10027
    3 Department of Applied Physics and Applied Mathematics, Columbia University, New York, New York 10027 and Nanotechnology Center for Mechanics in Regenerative Medicine, Columbia University, New York, New York 10027
    4 Department of Mechanical Engineering, Columbia University, New York, New York 10027 and Nanotechnology Center for Mechanics in Regenerative Medicine, Columbia University, New York, New York 10027
    a) Electronic mail: sw2128@columbia.edu
    J. Vac. Sci. Technol. B 27, 3088 (2009); http://dx.doi.org/10.1116/1.3259953
/content/avs/journal/jvstb/27/6/10.1116/1.3259953
http://aip.metastore.ingenta.com/content/avs/journal/jvstb/27/6/10.1116/1.3259953
View: Figures

Figures

Image of FIG. 1.
FIG. 1.

(a) Scanning electron micrograph of the PDMS posts. The diameter of the posts is and the pitch is . The height of the posts is . The stiffness of the post is about AAA. (b) Bright-field micrograph of the spreading of mouse embryonic fibroblast cell on the pillar substrate.

Image of FIG. 2.
FIG. 2.

Schematic of the process flow for fabricating arrays of the PDMS posts with functionalized Au on the top surface.

Image of FIG. 3.
FIG. 3.

(a) SEM image of holes in the silicon substrate. The diameter of the holes is and the pitch size is . The height of the hole is . (b) SEM image of final Si mold with a thin layer of Au and Ti at the bottom of the holes.

Image of FIG. 4.
FIG. 4.

Scanning electron micrograph of Au-tipped PDMS pillars. The tops of the pillars can be selectively chemical functionalized.

Image of FIG. 5.
FIG. 5.

Epifluorescence image of the tip of the functionalized pillars with diameter of and center-to-center distance of . The tops of the pillars are coated homogeneously with fluorescently labeled fibronectin and there is no signal from the bottom of the pillars.

Image of FIG. 6.
FIG. 6.

Image of the bottom of the pillars using confocal laser scanning microscopy. (a) The fluorescence signal of functionalized pillars decreases as along the length of the pillar, indicating that signal is coming from the top of pillars. (b) Rings of fluorescence signal around the sidewall of pillars in a nonfunctionalized substrate, indicating the complete coating of the sidewall.

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/content/avs/journal/jvstb/27/6/10.1116/1.3259953
2009-12-04
2014-04-17
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Gold-tipped elastomeric pillars for cellular mechanotransduction
http://aip.metastore.ingenta.com/content/avs/journal/jvstb/27/6/10.1116/1.3259953
10.1116/1.3259953
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