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Protein patterning on the micro- and nanoscale by thermal nanoimprint lithography on a new functionalized copolymer
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10.1116/1.3264687
/content/avs/journal/jvstb/27/6/10.1116/1.3264687
http://aip.metastore.ingenta.com/content/avs/journal/jvstb/27/6/10.1116/1.3264687
View: Figures

Figures

Image of FIG. 1.
FIG. 1.

Scheme showing the manufacturing process used for the combination of protein adsorption and nonprotein adsorption surfaces on the micro- and nanoscale. (a) Polymer deposition, (b) imprinting, (c) residual layer etching, (d) deposition, and (e) streptavidin adsorption.

Image of FIG. 2.
FIG. 2.

Scheme of the patterned substrates on the microscale. Five different grating periods among 3.3 and and equal line and space, each one of them with size of . , , , , and .

Image of FIG. 3.
FIG. 3.

(Color online) Fluorescence detection of streptavidin marked with the fluorescent label Alexa-Fluor 488 on (a) gratings with periods and equal lines and spaces and (b) 200 nm width lines spaced some micrometers.

Image of FIG. 4.
FIG. 4.

(Color online) Absorbance of light at 450 nm for the sandwich type structure for the IgG detection as the immunoassay is cultivated on (1) streptavidin and BSA protein on the copolymer, (2) BSA protein on the copolymer, (3) BSA on blank silicon, (4) on blank silicon, and (5) protein on blank silicon. Streptavidin concentration and BSA concentration for all the enzymatic reactions.

Image of FIG. 5.
FIG. 5.

(Color online) Fluorescence detection showing the presence of IgG in a patterned substrate comprised of grating periods and equal linewidths and spaces for the immunoassay proposed with (a) pure IgG (1mg/ml) and (b) diluted IgG (1:1000 or ).

Image of FIG. 6.
FIG. 6.

(Color online) Averaged signal measurements of the fluorescence intensity as a function of the concentration of the IgG in the samples for a substrate comprised of alternative microstructures of period and equal lines and spaces of polymer and , respectively.

Image of FIG. 7.
FIG. 7.

(Color online) Averaged signal measurements of the fluorescence intensity as a function of the concentration of IgG in the samples for a micropatterned imprinted polymer with period gratings of .

Image of FIG. 8.
FIG. 8.

(Color online) Curve of the IgG sandwich assay for the experimental data of Fig. 7 for imprinted polymer samples with period gratings of .

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/content/avs/journal/jvstb/27/6/10.1116/1.3264687
2009-11-19
2014-04-16
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Protein patterning on the micro- and nanoscale by thermal nanoimprint lithography on a new functionalized copolymer
http://aip.metastore.ingenta.com/content/avs/journal/jvstb/27/6/10.1116/1.3264687
10.1116/1.3264687
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