Skip to main content
banner image
No data available.
Please log in to see this content.
You have no subscription access to this content.
No metrics data to plot.
The attempt to load metrics for this article has failed.
The attempt to plot a graph for these metrics has failed.
The full text of this article is not currently available.
/content/avs/journal/jvstb/34/6/10.1116/1.4961591
1.
M. E. Spira and A. Hai, Nat. Nanotechnol. 8, 83 (2013).
http://dx.doi.org/10.1038/nnano.2012.265
2.
M. E. J. Obien, K. Deligkaris, T. Bullmann, D. J. Bakkum, and U. Frey, Front. Neurosci. 8, 423 (2015).
http://dx.doi.org/10.3389/fnins.2014.00423
3.
A. M. Hopkins, E. DeSimone, K. Chwalek, and D. L. Kaplan , Prog. Neurobiol. 125, 1 (2015).
http://dx.doi.org/10.1016/j.pneurobio.2014.11.003
4.
S. Shipp, Curr. Biol. 17, R443 (2007).
http://dx.doi.org/10.1016/j.cub.2007.03.044
5.
D. Huh, G. A. Hamilton, and D. E. Ingber, Trends Cell Biol. 21, 745 (2011).
http://dx.doi.org/10.1016/j.tcb.2011.09.005
6.
M. Y. Laura, N. D. Leipzig, and M. S. Shoichet, Mater. Today 11, 36 (2008).
http://dx.doi.org/10.1016/S1369-7021(08)70088-9
7.
X. Zhang, S. Prasad, S. Niyogi, A. Morgan, M. Ozkan, and C. S. Ozkan, Sens. Actuator, B 106, 843 (2005).
http://dx.doi.org/10.1016/j.snb.2004.10.039
8.
H. Francisco, B. B. Yellen, D. S. Halverson, G. Friedman, and G. Gallo, Biomaterials 28, 3398 (2007).
http://dx.doi.org/10.1016/j.biomaterials.2007.04.015
9.
M. D. Tang-Schomer et al., Proc. Natl. Acad. Sci. U.S.A. 111, 13811 (2014).
http://dx.doi.org/10.1073/pnas.1324214111
10.
J-P. Frimat, S. Xie, A. Bastiaens, F. Wolbers, J. den Toonder, and R. Luttge, J. Vac. Sci. Technol., B 33, 06F902 (2015).
http://dx.doi.org/10.1116/1.4931636
11.
B. Schurink, J. W. Berenschot, R. M. Tiggelaar, and R. Luttge, Microelectron. Eng. 144, 12 (2015).
http://dx.doi.org/10.1016/j.mee.2015.01.027
12.
B. Schurink, “Microfabrication and microfluidics for 3D brain-on-chip,” Ph.D. thesis ( University of Twente/Gildeprint, Enschede, The Netherlands, 2016).
13.
S. Xie and R. Luttge, Microelectron. Eng. 124, 30 (2014).
http://dx.doi.org/10.1016/j.mee.2014.04.012
14.
S. Xie, B. Schurink, F. Wolbers, G. Hassink, and R. Luttge, J. Vac. Sci. Technol., B 32, 06FD03 (2014).
http://dx.doi.org/10.1116/1.4900420
15.
H. H. Solak, C. Dais, and F. Clube , Opt. Express 19, 10686 (2011).
http://dx.doi.org/10.1364/OE.19.010686
16.
T. Uhrman et al., “ New optical lithography method for advanced light extraction in LEDs,” LED Professional, Issue No. 37 (2013).
17.
H. Le-The, E. Berenschot, R. M. Tiggelaar, N. R. Tas, A. van den Berg, and J. C. T. Eijkel, Proceedings of the 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (uTAS), Dublin, Ireland, 9–13 October (2016).
18.
S. Gupta and C. F. Lyons, U.S. patent 5,807,790 (15 September 1998).
19.
Y. X. Li, P. J. French, and R. F. Wolffenbuttel, J. Vac. Sci. Technol., B 13, 2008 (1995).
http://dx.doi.org/10.1116/1.588124
20.
See supplementary material at http://dx.doi.org/10.1116/1.4961591 for details of the analysis of the aligned outgrowths on the nanoscaffold with Fast Frontier Transform algorithm.[Supplementary Material]
http://aip.metastore.ingenta.com/content/avs/journal/jvstb/34/6/10.1116/1.4961591
Loading
/content/avs/journal/jvstb/34/6/10.1116/1.4961591
Loading

Data & Media loading...

Loading

Article metrics loading...

/content/avs/journal/jvstb/34/6/10.1116/1.4961591
2016-08-26
2016-09-30

Abstract

Commercial microelectrode arrays (MEAs) for neuroelectrophysiology studies rely on conventional two dimensional (2D) neuronal cultures that are seeded on the planar surface of such MEAs and thus form a random neuronal network. The cells attaching on these types of surfaces grow in 2D and lose their native morphology, which may also influence their neuroelectrical behavior. Besides, a random neuronal network formed on this planar surface also lacks comparison to the state of brain tissue. In order to improve the present MEA platform with the above mentioned concerns, in this paper, the authors introduce a three dimensional platform for neuronal cell culturing, where a linear nanoscaffold is patterned on a microsieve array by displacement Talbot lithography (DTL) and reactive ion etching. Good pattern uniformity is achieved by the DTL method on the topographically prepatterned nonflat surface of the microsieve array. Primary cortical cells cultured on the nanopatterned microsieve array show an organized network due to the contact guidance provided by the nanoscaffold, presenting 47% of the total outgrowths aligning with the nanogrooves in the observed view of field. Hence, the authors state that this nanopatterned microsieve array can be further integrated into microsieve-based microelectrode arrays to realize an advanced Brain-on-Chip model that allows us to investigate the neurophysiology of cultured neuronal networks with specifically organized architectures.

Loading

Full text loading...

/deliver/fulltext/avs/journal/jvstb/34/6/1.4961591.html;jsessionid=QW4valMk62cMgl9TCqMAmV9e.x-aip-live-06?itemId=/content/avs/journal/jvstb/34/6/10.1116/1.4961591&mimeType=html&fmt=ahah&containerItemId=content/avs/journal/jvstb
true
true

Access Key

  • FFree Content
  • OAOpen Access Content
  • SSubscribed Content
  • TFree Trial Content
752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
/content/realmedia?fmt=ahah&adPositionList=
&advertTargetUrl=//oascentral.aip.org/RealMedia/ads/&sitePageValue=jvstb.avspublications.org/34/6/10.1116/1.4961591&pageURL=http://scitation.aip.org/content/avs/journal/jvstb/34/6/10.1116/1.4961591'
Right1,Right2,Right3,