banner image
No data available.
Please log in to see this content.
You have no subscription access to this content.
No metrics data to plot.
The attempt to load metrics for this article has failed.
The attempt to plot a graph for these metrics has failed.
Cellular reactions toward nanostructured silicon surfaces created by laser ablation
Rent this article for
Access full text Article
1. M. H. You, M. K. Kwak, D. H. Kim, K. Kim, A. Levchenko, D. Y. Kim, and K. Y. Suh, “ Synergistically enhanced osteogenic differentiation of human mesenchymal stem cells by culture on nanostructured surfaces with induction media,” Biomacromolecules 11, 18561862 (2010).
2. J. Reichert, S. Brückner, H. Bartelt, and K. D. Jandt, “ Tuning cell adhesion on PTFE surfaces by laser induced microstructures,” Adv. Eng. Mater. 9, 11041113 (2007).
3. R. G. Flemming, C. J. Murphy, G. A. Abrams, S. L. Goodman, and P. F. Nealey, “ Effects of synthetic micro- and nano-structured surfaces on cell behavior,” Biomaterials 20, 573588 (1998).
4. U. Meyer, A. Büchter, H. P. Wiesmann, U. Joos, and D. B. Jones, “ Basic reactions of osteoblasts on structured material surfaces,” Eur. Cells Mater. 9, 3949 (2005).
5. Y. Hu, K. Cai, Z. Luo, R. Zhang, L. Yang, L. Deng, and K. D. Jandt, “ Surface mediated in situ differentiation of mesenchymal stem cells on gene-functionalized titanium films fabricated by layer-by-layer technique,” Biomaterials 30, 36263635 (2009).
6. M. J. Dalby, M. O. Riehle, H. Johnstone, S. Affrossman, and A. S. G. Curtis, “ In vitro reaction of endothelial cells to polymer demixed nanotopography,” Biomaterials 23, 29452954 (2002).
7. C. Hallgren, H. Reimers, D. Chakarov, J. Gold, and A. Wennerberg, “ An in vivo study of bone response to implants topographically modified by laser micromachining,” Biomaterials 24, 701710 (2003).
8. S. Petronis, C. Gretzer, B. Kasemo, and J. Gold, “ Model porous surfaces for systematic studies of material-cell interactions,” J. Biomed. Mater. Res. 66A, 707721 (2003).
9. J. M. Rice, J. A. Hunt, J. A. Gallagher, P. Hanarp, D. S. Sutherland, and J. Gold, “ Quantitative assessment of the response of primary derived human osteoblasts and macrophages to a range of nanotopography surfaces in a single culture model in vitro,” Biomaterials 24, 47994818 (2003).
10. B. D. Boyan, S. Lossdörfer, L. Wang, G. Zhao, C. H. Lohmann, D. L. Cochran, and Z. Schwartz, “ Osteoblasts generate an osteogenic microenvironment when grown on surfaces with rough microtopographies,” Eur. Cells Mater. 6, 2227 (2003).
11. H. O. Schwartz Fo, A. B. Novaes, L. M. S. de Castro, A. L. Rosa, and P. T. de Oliveira, “ In vitro osteogenesis on a microstructured titanium surface with additional submicron-scale topography,” Clin. Oral Implants Res. 18, 333344 (2007).
12. N. Walter, C. Selhuber, H. Kessler, and J. P. Spatz, “ Cellular unbinding forces of initial adhesion processes on nanopatterned surfaces probed with magnetic tweezers,” Nano Lett. 6, 398402 (2006).
13. B. Zhu, Q. Lu, J. Yin, J. Hu, and Z. Wang, “ Effects of laser-modified polystyrene substrate on CHO cell growth and alignment,” J. Biomed. Mater. Res., Part B: Appl. Biomater. 70B, 4348 (2004).
14. E. Rebollar, I. Frischauf, M. Olbrich, T. Peterbauer, S. Hering, J. Preiner, P. Hinterdorfer, C. Romanin et al., “ Proliferation of aligned mammalian cells on laser-nanostructured polystyrene,” Biomaterials 29, 17961806 (2008).
15. M. J. P. Biggs, R. G. Richards, and M. J. Dalby, “ Nanotopographical modification: a regulator of cellular function through focal adhesions,” Nanomed. Nanotechnol. Biol. Med. 6, 619633 (2010).
16. C. Y. Tay, S. A. Irvine, F. Y. C. Boey, L. P. Tan, and S. Venkatraman, “ Micro-/nano-engineered cellular responses for soft tissue engineering and biomedical applications,” Small 7, 13611378 (2011).
17. B. Tan and K. Venkatakrishnan, “ A femtosecond laser-induced periodical surface structure on crystalline silicon,” J. Micromech. Microeng. 16, 10801085 (2006).
18. J. Bonse, A. Rosenfeld, and J. Kruger, “ On the role of surface plasmon polaritons in the formation of laser-induced periodic surface structures upon irradiation of silicon by femtosecond-laser pulses,” J. Appl. Phys. 106(10 ), 104910 (2009).
19. R. Le Harzic, D. Dörr, D. Sauer, F. Stracke, and H. Zimmermann, “ Generation of high spatial frequency ripples on silicon under ultrashort laser pulses irradiation,” Appl. Phys. Lett. 98, 211905 (2011).
20. R. Le Harzic, D. Dörr, D. Sauer, M. Neumeier, M. Epple, H. Zimmermann, and F. Stracke, “ Formation of periodic nanoripples on silicon and germanium induced by femtosecond laser pulses,” Phys. Procedia 12, 2936 (2011).
21. R. Le Harzic, D. Dörr, D. Sauer, M. Neumeier, M. Epple, H. Zimmermann, and F. Stracke, “ Large-area, uniform, high-spatial-frequency ripples generated on silicon using a nanojoule-femtosecond laser at high repetition rate,” Opt. Lett. 36, 229231 (2011).
22. A. Menendez-Manjon, J. Jakobi, K. Schwabe, J. K. Krauss, and S. Barcikowski, “ Mobility of nanoparticles generated by femtosecond laser ablation in liquids and its application to surface patterning,” J. Laser Micro/Nanoeng. 4, 9599 (2009).
23. A. R. Boccaccini, S. Keim, R. Ma, Y. Li, and I. Zhitomirsky, “ Electrophoretic deposition of biomaterials,” J. R. Soc., Interface 7(Suppl 5), S581S613 (2010).
24. M. Epple, M. Neumeier, D. Dörr, R. LeHarzic, D. Sauer, F. Stracke, and H. Zimmermann, “ Electrophoretic deposition of calcium phosphate nanoparticles on a nanostructured silicon surface,” Materialwiss. Werkstofftechn. 42, 5054 (2011).
View: Figures


Image of FIG. 1.

Click to view

FIG. 1.

A silicon surface with HFSL of 125 nm mean spacing generated with an illumination wavelength of 800 nm.

Image of FIG. 2.

Click to view

FIG. 2.

Top left: hMSC seeded on a silicon substrate with an HFSL-structured section after 48 h of cultivation (dark area). The cells have mostly left the structured area (light microscopy, calcein fluorescence/reflection overlay, cells appear green). Other images: SEM images of L929 fibroblasts after 24 h of cultivation on structured and unstructured areas of silicon substrates (chessboard geometry). The bright areas are nanostructured silicon; the dark areas are unstructured silicon. The cells density on the unstructured areas was significantly higher than that on the nanostructured areas. The image on the bottom right shows an L929 fibroblast on a structured area with numerous filopodia on focal adhesion points (note that the HSFLs are not resolved at this magnification).

Image of FIG. 3.

Click to view

FIG. 3.

A monolayer of calcium phosphate nanoparticles (diameter 75 nm) on structured silicon in two different magnifications. The underlying HSFL ripple structure is still visible.

Image of FIG. 4.

Click to view

FIG. 4.

HeLa cells cultivated on a structured silicon substrate, originally coated with a monolayer of calcium phosphate nanoparticles. The ripple structure was still visible below the cell. The cells were well spread out on the nanostructured surface.


Article metrics loading...



Silicon wafers were structured with a femtosecond laser on the cm2 scale with high spatial frequency laser-induced periodic surface structures. These areas are characterized by regular parallel ripples with a period of the order of 100 nm. The particular ripple spacing is determined by the illumination wavelength of the tunable femtosecond laser. The cellular reaction to the structuredsilicon wafers and to the same materials, coated with calcium phosphate nanoparticles by electrophoretic deposition, was studied using L929 fibroblasts, human mesenchymal stem cells, and epithelial cells. The cells adhered uniformly to structured and unprocessed areas after seeding but significantly preferred the unstructured silicon after 48 h. This behavior disappeared after coating the structuredsurface with calcium phosphate nanoparticles.


Full text loading...

This is a required field
Please enter a valid email address
752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Cellular reactions toward nanostructured silicon surfaces created by laser ablation