Activity of single ion channel proteins detected with a planar microstructure
Appl. Phys. Lett. 81, 4865 (2002); doi:10.1063/1.1531228
Issue Date: 16 December 2002
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We present recordings of currents mediated by single ion channel proteins obtained using planar, microstructured glass chips. In these chips, pores with diameters of 12 µm are produced by ion track etching and are used for patch clamping instead of using the classical micropipette. Our results represent success in using such devices to record from single channels in cell membranes. The planar chip greatly enhances the accessibility of the ion channel containing membrane and can serve as a workbench for experiments on single ion channels using combinations of patch clamp current recording with other single molecule techniques. ©2002 American Institute of Physics.
| History: | Received 3 October 2002; accepted 30 October 2002 |
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KEYWORDS and PACS
- 85.65.+h
Electronic and magnetic devices; microelectronics Molecular electronic devices - 87.14.Ee
Biological and medical physics Biomolecules: types Proteins - 87.15.Vv
Biological and medical physics Biomolecules: structure and physical properties Diffusion - 82.37.Rs
Physical chemistry and chemical physics Single molecule kinetics Single molecule manipulation of proteins and other biological molecules - YEAR: 2002
RELATED DATABASES
PUBLICATION DATA
0003-6951 (print)
1077-3118 (online)
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