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Activity of single ion channel proteins detected with a planar microstructure

Appl. Phys. Lett. 81, 4865 (2002); doi:10.1063/1.1531228

Issue Date: 16 December 2002

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Niels Fertig
Center for NanoScience and Physiologisches Institut, Ludwig-Maximilians-Universität, Pettenkoferstraße12, 80336 München, Germany
Nanion Technologies GmbH, Schellingstr. 4/IV, 80799 München, Germany


Michèle Klau
Center for NanoScience and Physiologisches Institut, Ludwig-Maximilians-Universität, Pettenkoferstraße12, 80336 München, Germany

Michael George
Center for NanoScience and Psysiologisches Institut, Ludwig-Maximilians-Universität, Pettenkoferstraße12, 80336 München, Germany
Nanion Technologies GmbH, Schellingstr. 4/IV, 80799 München, Germany


Robert H. Blick
Center for NanoScience and Sektion Physik, Ludwig-Maximilians-Universität, Geschwister-Scholl-Platz 1, 80539 München, Germany

Jan C. Behrends
Center for NanoScience and Physiologisches Institut, Ludwig-Maximilians-Universität, Pettenkoferstraße12, 80336 München, Germany
We present recordings of currents mediated by single ion channel proteins obtained using planar, microstructured glass chips. In these chips, pores with diameters of 1–2 µm are produced by ion track etching and are used for patch clamping instead of using the classical micropipette. Our results represent success in using such devices to record from single channels in cell membranes. The planar chip greatly enhances the accessibility of the ion channel containing membrane and can serve as a workbench for experiments on single ion channels using combinations of patch clamp current recording with other single molecule techniques. ©2002 American Institute of Physics.
History: Received 3 October 2002; accepted 30 October 2002
Permalink: http://link.aip.org/link/?APPLAB/81/4865/1
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KEYWORDS and PACS

Keywords
PACS
  • 85.65.+h
    Electronic and magnetic devices; microelectronics Molecular electronic devices
  • 87.14.Ee
    Biological and medical physics Biomolecules: types Proteins
  • 87.15.Vv
    Biological and medical physics Biomolecules: structure and physical properties Diffusion
  • 82.37.Rs
    Physical chemistry and chemical physics Single molecule kinetics Single molecule manipulation of proteins and other biological molecules
  • YEAR: 2002

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PUBLICATION DATA

ISSN:
0003-6951 (print)   1077-3118 (online)
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REFERENCES (14)

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