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Microfluidic polymerase chain reaction

Appl. Phys. Lett. 93, 243901 (2008); doi:10.1063/1.3046789

Published 16 December 2008

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George Maltezos,1 Alvaro Gomez,2 Jiang Zhong,3 Frank A. Gomez,2 and Axel Scherer1
1Department of Electrical Engineering, California Institute of Technology, 1200 East California Boulevard, MS 200-36 Pasadena, California 91125, USA
2Department of Chemistry and Biochemistry, California State University, Los Angeles, 5151 State University Drive, Los Angeles, California 90032-8202, USA
3Keck School of Medicine, University of Southern California, Los Angeles, California 90089, USA
We implement microfluidic technology to miniaturize a thermal cycling system for amplifying DNA fragments. By using a microfluidic thermal heat exchanger to cool a Peltier junction, we have demonstrated rapid heating and cooling of small volumes of solution. We use a miniature K-type thermocouple to provide a means for in situ sensing of the temperature inside the microrefrigeration system. By combining the thermocouple, two power supplies controlled by a relay system, and computer automation, we reproduce the function of a commercial polymerase chain reaction thermal cycler and demonstrate amplification of a DNA sample of about 1000 base pairs. ©2008 American Institute of Physics
History: Received 4 July 2008; accepted 12 November 2008; published 16 December 2008
Permalink: http://link.aip.org/link/?APPLAB/93/243901/1
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KEYWORDS and PACS

Keywords
PACS
  • 87.80.Ek
    Mechanical and micromechanical techniques (biophysical research methods)
  • 07.20.Dt
    Thermometers
  • 87.14.gk
    DNA
  • YEAR: 2008

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PUBLICATION DATA

ISSN:
0003-6951 (print)   1077-3118 (online)
Publisher:
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REFERENCES (8)
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  1. K. B. Mullis, F. Ferre, and R. A. Gibbs, The Polymerase Chain Reaction (Birkhäuser, Boston, 1994).
  2. M. J. Adler, C. Coronel, E. Shelton, J. E. Seegmiller, and N. N. Dewji, Proc. Natl. Acad. Sci. U.S.A. 88, 16 (1991).
  3. http://users.ugent.be/~avierstr/principles/pcr.html.
  4. G. Maltezos, M. Johnston, and A. Scherer, Appl. Phys. Lett. 87, 154105 (2005).
  5. Z. Chunsun, X. Jinliang, W. Jianqin, and W. Hanping, Chin. J. Anal. Chem. 34, 8 (2006).
  6. X. Zhou, D. Liu, R. Zhong, Z. Dai, D. Wu, H. Wang, Y. Du, Z. Xia, L. Zhang, X. Mei, and B. Lin, Electrophoresis 25, 17 (2004).
  7. G. V. Kaigala, R. J. Huskins, J. Preiksaitis, X. Pang, L. M. Pilarski, and C. J. Backhouse, Electrophoresis 27, 3753 (2006).
  8. J. Liu, M. Enzelberger, and S. Quake, Electrophoresis 23, 10 (2002).

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