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Electrical detection of single-base DNA mutation using functionalized nanoparticles

Appl. Phys. Lett. 95, 073703 (2009); doi:10.1063/1.3152768

Published 17 August 2009

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Mohammud R. Noor,1,2 Swati Goyal,2,3 Shawn M. Christensen,4 and Samir M. Iqbal1,2,5
1Department of Electrical Engineering, The University of Texas at Arlington, Arlington, Texas 76019, USA
2Nanotechnology Research and Teaching Facility, The University of Texas at Arlington, Arlington, Texas 76019, USA
3Department of Bioengineering, The University of Texas at Arlington, Arlington, Texas 76019, USA
4Department of Biology, The University of Texas at Arlington, Arlington, Texas 76019, USA
5Joint Graduate Committee of Biomedical Engineering Program, University of Texas at Arlington and University of Texas Southwestern Medical Center at Dallas, Arlington, Texas 76019 USA
We report an electrical scheme to detect specific DNA. Engineered hairpin probe DNA are immobilized on a silicon chip between gold nanoelectrodes. Hybridization of target DNA to the hairpin melts the stem nucleotides. Gold nanoparticle-conjugated universal reporter sequence detects the open hairpins by annealing to the exposed stem nucleotides. The gold nanoparticles increase charge conduction between the electrodes. Specifically, we report on a hairpin probe designed to detect a medically relevant mutant form of the K-ras oncogene. Direct current measurements show three orders of magnitude increase in conductivity for as low as 2  fmol of target molecules. ©2009 American Institute of Physics
History: Received 25 March 2009; accepted 18 May 2009; published 17 August 2009
Permalink: http://link.aip.org/link/?APPLAB/95/073703/1
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KEYWORDS and PACS

Keywords
PACS
  • 87.85.Rs
    Nanotechnologies - applications in biomedical engineering
  • 87.15.Pc
    Electronic and electrical properties of biomolecules
  • 87.14.gk
    DNA
  • YEAR: 2009

PUBLICATION DATA

ISSN:
0003-6951 (print)   1077-3118 (online)
Publisher:
AIP is a member of CrossRef AIP

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