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Cerium oxide-chitosan based nanobiocomposite for food borne mycotoxin detection

Appl. Phys. Lett. 95, 173703 (2009); doi:10.1063/1.3249586

Published 29 October 2009

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Ajeet Kaushik,1,2 Pratima R. Solanki,1 M. K. Pandey,1 Sharif Ahmad,2 and Bansi D. Malhotra1,3
1Department of Science and Technology Centre on Biomolecular Electronics, National Physical Laboratory, New Delhi 110012, India
2Department of Chemistry, Materials Research Laboratory, Jamia Millia Islamia, New Delhi 110025, India
3Centre for Nano-Bioengineering and Spintronics, Chungnam National University, Daejeon 305-764, Republic of Korea
Cerium oxide nanoparticles (NanoCeO2) and chitosan (CH) based nanobiocomposite film deposited onto indium-tin-oxide coated glass substrate has been used to coimmobilize rabbit immunoglobin (r-IgGs) and bovine serum albumin (BSA) for food borne mycotoxin [ochratoxin-A (OTA)] detection. Electrochemical studies reveal that presence of NanoCeO2 increases effective electro-active surface area of CH-NanoCeO2/indium tin oxide (ITO) nanobiocomposite resulting in high loading of r-IgGs. BSA/r-IgGs/CH-NanoCeO2/ITO immunoelectrode exhibits improved linearity (0.25–6.0 ng/dl), detection limit (0.25 ng/dl), response time (25 s), sensitivity (18  µA/ng dl−1 cm−2), and regression coefficient (r2~0.997). ©2009 American Institute of Physics
History: Received 11 September 2009; accepted 21 September 2009; published 29 October 2009
Permalink: http://link.aip.org/link/?APPLAB/95/173703/1
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EPAPS

KEYWORDS and PACS

Keywords
PACS
  • 07.07.Df
    Sensors (chemical, optical, electrical, movement, gas, etc.); remote sensing
  • 82.47.Rs
    Electrochemical sensors
  • 82.80.Fk
    Electrochemical analytical methods
  • 87.80.Kc
    Electrochemical techniques (biophysical research methods)
  • 87.14.E-
    Proteins
  • 82.45.Fk
    Electrochemical electrodes
  • YEAR: 2009

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ISSN:
0003-6951 (print)   1077-3118 (online)
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REFERENCES (17)
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