Applied Physics Letters
Feedback | Help | Exit
Applied Physics Letters
Search:
   
 
 
 
Previous Article
Energy band-alignment of a multimetal-layer gated metal-oxide-semiconductor structure
The energy band-alignment of a multimetal-layer gated metal-oxide-semiconductor structure is investigated. An analytical expression for the Fermi level of the metal stack is derived. It is shown that ...
Next Article
Individual charge-trapping dislocations in an ionic insulator
Insulating oxide materials find widespread technological applications where how their inside dislocations behave are known to influence or control performance. Here we demonstrate, by first-principles...

Soluble state high resolution atomic force microscopy study of Alzheimer's beta-amyloid oligomers

Appl. Phys. Lett. 95, 183701 (2009); doi:10.1063/1.3251779

Published 6 November 2009

You are not logged in to this journal. Log in

Gajendra S. Shekhawat,1 Mary P. Lambert,2 Saurabh Sharma,1 Pauline T. Velasco,2 Kirsten L. Viola,2 William L. Klein,2 and Vinayak P. Dravid1
1Department of Materials Science and Engineering, International Institute for Nanotechnology and NUANCE Center, Northwestern University, Evanston, Illinois 60004, USA
2Department of Neurobiology and Physiology, Northwestern University, Evanston, Illinois 60208, USA
We report here the direct observation of high resolution structures of assemblies of Alzheimer beta-amyloid oligomers and monomers using liquid atomic force microscopy (AFM). Visualization of nanoscale features of Abeta oligomers (also known as ADDLs) was carried out in tapping mode AFM in F12 solution. Our results indicate that ADDL preparations exist in solution primarily as a mixture of monomeric peptides and higher molecular mass oligomers. Our study clearly reveals that the size and shape of these oligomer aggregates exhibit a pronounced dependence on concentration. These studies show that wet AFM enables direct assessment of oligomers in physiological fluids and suggests that this method may be developed to visualize Abeta oligomers from human fluids. ©2009 American Institute of Physics
History: Received 23 July 2009; accepted 29 September 2009; published 6 November 2009
Permalink: http://link.aip.org/link/?APPLAB/95/183701/1
BUY THIS ARTICLE   (US$24)
Download HTML Download Sectioned HTML Download PDF (354 kB) View Cart

KEYWORDS and PACS

Keywords
PACS
  • 87.64.Dz
    Scanning tunneling and atomic force microscopy in biophysics and medical physics
  • 87.19.xr
    Degenerative diseases
  • 87.14.ef
    Peptides
  • 87.14.em
    Fibrils (proteins)
  • 87.15.bk
    Structure of biomolecular aggregates
  • 87.80.-y
    Biophysical techniques (research methods)
  • YEAR: 2009

PUBLICATION DATA

ISSN:
0003-6951 (print)   1077-3118 (online)
Publisher:
AIP is a member of CrossRef AIP

REFERENCES (23)
View in Separate Window/Tab
For access to fully linked references, you need to log in. For access to fully linked references, you need to Log in.
  1. M. P. Lambert, A. K. Barlow, B. A. Chromy, E. Edwards, R. Freed, M. Liosatos, T. E. Morgan, I. Rozovsky, B. Trommer, K. L. Viola, P. Wals, C. Zhang, C. E. Finchs, G. A. Kraff, and W. L. Klein, Proc. Natl. Acad. Sci. U.S.A. 95, 6448 (1998).
  2. D. Selkoe, Science 275, 630 (1997).
  3. B. A. Yanker, Neuron 16, 921 (1996).
  4. T. Wisniewski, J. Ghiso, and B. Frangione, Neurobiol. Dis. 4, 313 (1997).
  5. C. Geula, C. -K. Wu, D. Saroff, A. Lorenzo, M. Yuan, and B. A. Yanker, Nat. Med. 4, 827 (1998).
  6. W. Klein, G. A. Krafft, and C. E. Finch, Trends Neurosci. 24, 219 (2001).
  7. W. L. Klein, F. G. De Felice, P. N. Lacor, M. P. Lambert, and W. -Q. Zhao, in Synaptic Plasticity and the Mechanism of Alzheimer's Disease, edited by D. J. Selkoe, A. Triller, and Y. Christen (Springer, Berlin, 2008), pp. 103–132.
  8. F. G. De Felice, M. N. N. Vieira, T. R. Bomfim, H. Decker, P. T. Velasco, M. P. Lambert, K. L. Viola, W. -Q. Zhao, S. T. Ferreira, and W. L. Klein, Proc. Natl. Acad. Sci. U.S.A. 106, 1971 (2009).
  9. P. N. Lacor, M. C. Buniel, P. W. Furlow, A. S. Clemente, P. T. Velasco, M. Wood, K. L. Viola, and W. L. Klein, J Neurosci. 27, 796 (2007).
  10. O. Boutaud, T. J. Montine, L. Chang, W. L. Klein, and J. A. Oates, J. Neurochem. 96, 917 (2006).
  11. Y. Gong, L. Chang, K. L. Viola, P. N. Lacor, M. P. Lambert, C. E. Finch, G. A. Krafft, and W. L. Klein, Proc. Natl. Acad. Sci. U.S.A. 100, 10417 (2003).
  12. S. Lesne, M. T. Koh, L. Kotilinek, R. Kayed, C. G. Glabe, A. Yang, M. Gallagher, and K. H. Ashe, Nature (London) 440, 352 (2006).
  13. B. A. Chromy, R. J. Nowak, M. P. Lambert, K. L. Viola, L. Chang, P. T. Velasco, B. W. Jones, S. J. Fernandez, P. N. Lacor, P. Horowitz, C. E. Finch, G. A. Krafft, and W. L. Klein, Biochemistry 42, 12749 (2003).
  14. C. Schladitz, E. P. Vieira, H. Hermel, and H. Mohwald, Biophys. J. 77, 3305 (1999).
  15. O. Gursky and S. Aleshkov, Biochim. Biophys. Acta 1476, 621 (2000).
  16. H. K. Narang, J. Neuropathol. Exp. Neurol. 39, 621 (1980).
  17. M. A. Kurt, D. C. Davies, and M. Kidd, Exp. Neurol. 158, 221 (1999).
  18. K. Lundmark, G. T. Westermark, A. Olse'n, and P. Westermark, Proc. Natl. Acad. Sci. U.S.A. 102, 6098 (2005).
  19. C. Bustamante, D. A. Erie, and D. Keller, Curr. Opin. Struct. Biol. 4, 750 (1994).
  20. D. Walsh, D. M. Hartley, G. B. Benedek, M. M. Condron, and D. B. Teplow, J. Biol. Chem. 272, 22364 (1997).
  21. J. Harper, S. S. Wong, C. M. Leiber, and P. T. Lansbury, Chem. Biol. 4, 119 (1997).
  22. V. Rangachari, B. D. Moore, D. K. Reed, L. K. Sonoda, A. W. Bridges, E. Conboy, D. Hartigan, and T. L. Rosenberry, Biochemistry 46, 12451 (2007).
  23. W. L. Klein, Neurochem. Int. 41, 345 (2002).

CITING ARTICLES
For access to citing articles, you need to log in.
For access to citing articles, you need to Log in.


Copyright © American Institute of Physics