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An integrated dielectrophoretic chip for continuous bioparticle filtering, focusing, sorting, trapping, and detecting

Biomicrofluidics 1, 021503 (2007); doi:10.1063/1.2723669

Published 10 May 2007

I-Fang Cheng and Hsien-Chang Chang
Institute of Nanotechnology and Microsystem Engineering, Institute of Biomedical Engineering, National Cheng Kung University, Tainan, Taiwan, Republic of China

Diana Hou and Hsueh-Chia Chang
Center for Microfluidics and Medical Diagnostics, Department of Chemical and Biomolecular Engineering, University of Notre Dame, Notre Dame, Indiana 46556
Multi-target pathogen detection using heterogeneous medical samples require continuous filtering, sorting, and trapping of debris, bioparticles, and immunocolloids within a diagnostic chip. We present an integrated AC dielectrophoretic (DEP) microfluidic platform based on planar electrodes that form three-dimensional (3D) DEP gates. This platform can continuously perform these tasks with a throughput of 3  µL/min. Mixtures of latex particles, Escherichia coli Nissle, Lactobacillus, and Candida albicans are sorted and concentrated by these 3D DEP gates. Surface enhanced Raman scattering is used as an on-chip detection method on the concentrated bacteria. A processing rate of 500 bacteria was estimated when 100  µl of a heterogeneous colony of 107 colony forming units /ml was processed in a single pass within 30  min. ©2007 American Institute of Physics
History: Received 21 December 2006; accepted 1 March 2007; published 10 May 2007
Permalink: http://link.aip.org/link/?BIOMGB/1/21503/1
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KEYWORDS and PACS

Keywords
PACS
  • 87.80.-y
    Biological techniques and instrumentation; biomedical engineering
  • 87.19.Nn
    Electrophysiology (higher organisms)
  • 85.85.+j
    Micro- and nano-electromechanical systems (MEMS/NEMS) and devices
  • 82.45.Tv
    Bioelectrochemistry
  • 47.85.Np
    Fluidics (applied)
  • 07.07.Df
    Sensors (chemical, optical, electrical, movement, gas, etc.); remote sensing
  • YEAR: 2007

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PUBLICATION DATA

ISSN:
1932-1058 (online)
Publisher:
AIP is a member of CrossRef AIP

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