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Rapid detection of natriuretic peptides by a microfluidic LabChip analyzer with DNA aptamers: Application of natriuretic peptide detection

Biomicrofluidics 3, 034101 (2009); doi:10.1063/1.3194283

Published 30 July 2009

Ming-Cheng Lin,1,2 Jiraporn Nawarak,3 Tai-Yuan Chen,4 Hsien-Yu Tsai,3,5 Jung-Feng Hsieh,6 Supachok Sinchaikul,3 and Shui-Tein Chen3,5
1Department of Internal Medicine, Division of Cardiology, Chung Shan Medical University Hospital, Taichung 40201, Taiwan
2School of Medicine, Chung Shan Medical University, Taichung 40201, Taiwan
3Institute of Biological Chemistry, Academia Sinica, 128 Academia Rd., Sec. 2, Nankang, Taipei 11529, Taiwan
4Department of Medical Research, Clinical Genomics and Proteomics Core Laboratory, Chang Gung Memorial Hospital at Kaohsiung, Kaohsiung County 833, Taiwan
5Institute of Biochemical Sciences, College of Life Science, National Taiwan University, Taipei 10617, Taiwan
6Department of Food Science, Fu Jen Catholic University, Xin Zhuang, Taipei 242, Taiwan
Rapid detection of brain natriuretic peptide (BNP) concentration can be used for the diagnosis of acute heart failure and for the evaluation of the effectiveness of a clinical therapy. We used the systematic evolution of ligands by exponential enrichment method to develop DNA aptamers for BNP whose sequences were determined by cloning method and consensus sequence analysis. A total of eight conserved sequences was identified. By combining the fluorescent-labeled aptamers with fast protein lab-on-chip analysis, we could achieve quantification of BNP concentrations with high speed, sensitivity, and specificity. ©2009 American Institute of Physics
History: Received 12 May 2009; accepted 13 July 2009; published 30 July 2009
Permalink: http://link.aip.org/link/?BIOMGB/3/34101/1
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1932-1058 (online)
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