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A microfluidic mixing system for single-molecule measurements

Rev. Sci. Instrum. 80, 055105 (2009); doi:10.1063/1.3125643

Published 14 May 2009

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Shawn H. Pfeil,1 Charles E. Wickersham,1 Armin Hoffmann,2 and Everett A. Lipman1
1Department of Physics, University of California, Santa Barbara, California 93106, USA
2Biochemisches Institut, Universität Zürich, Winterthurerstrasse 190, 8057 Zürich, Switzerland
This article describes the design and fabrication of a microfluidic mixing system optimized for ultrasensitive optical measurements. Channels are replica-molded in polydimethylsiloxane elastomer and sealed with fused-silica coverglass. The resulting devices have broad chemical compatibility and extremely low fluorescence background, enabling measurements of individual molecules under well-characterized nonequilibrium conditions. Fluid delivery and pressure connections are made using an interface that allows for rapid assembly, rapid sample exchange, and modular device replacement while providing access for high numerical aperture optics. ©2009 American Institute of Physics
History: Received 23 December 2008; accepted 7 April 2009; published 14 May 2009
Permalink: http://link.aip.org/link/?RSINAK/80/055105/1
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KEYWORDS and PACS

Keywords
PACS
  • 07.10.Cm
    Micromechanical devices and systems
  • 33.50.Dq
    Molecular fluorescence and phosphorescence spectra
  • 37.20.+j
    Atomic and molecular beam sources and techniques
  • 85.85.+j
    Micro- and nano-electromechanical systems (MEMS/NEMS) and devices
  • 42.15.Eq
    Optical system design
  • 42.82.Cr
    Optical fabrication techniques; lithography, pattern transfer (integrated optics)
  • YEAR: 2009

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PUBLICATION DATA

ISSN:
0034-6748 (print)   1089-7623 (online)
Publisher:
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