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Confocal single molecule fluorescence spectroscopy in ultrahigh vacuum

Rev. Sci. Instrum. 80, 103101 (2009); doi:10.1063/1.3238505

Published 6 October 2009

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Michael L. Blumenfeld, Brandon S. Tackett, Laura K. Schirra, Jason M. Tyler, and Oliver L. A. Monti
Department of Chemistry, The University of Arizona, 1306 E. University Blvd., Tucson, Arizona 85721, USA
We have constructed an ultrahigh vacuum confocal fluorescence microscope with the purpose of performing single molecule spectroscopy under highly defined conditions. The microscope is designed for high stability while affording the capability of sample preparation, sample transfer, and optical detection in ultrahigh vacuum. It achieves near-diffraction-limited performance and allows the observation of single molecule fluorescence dynamics over extended periods of time. The design of the microscope is discussed in detail and the performance is demonstrated with single molecule fluorescence images and trajectories of N,N[prime]-dibutylperylene-3,4,9,10-dicarboxyimide deposited onto several different surfaces. This instrument further enhances the array of available surface science techniques, permitting spectroscopic investigations of molecule-surface interactions at the single molecule level and on insulating surfaces. ©2009 American Institute of Physics
History: Received 25 July 2009; accepted 5 September 2009; published 6 October 2009
Permalink: http://link.aip.org/link/?RSINAK/80/103101/1
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KEYWORDS and PACS

Keywords
PACS
  • 07.60.Pb
    Conventional optical microscopes
  • 07.60.Rd
    Visible and ultraviolet spectrometers
  • 07.30.-t
    Vacuum apparatus
  • 33.50.Dq
    Molecular fluorescence and phosphorescence spectra
  • 34.35.+a
    Interactions of atoms and molecules with surfaces
  • YEAR: 2009

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PUBLICATION DATA

ISSN:
0034-6748 (print)   1089-7623 (online)
Publisher:
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REFERENCES (24)
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  1. J. J. Macklin, J. K. Trautman, T. D. Harris, and L. E. Brus, Science 272, 255 (1996).
  2. E. Barkai, Y. J. Jung, and R. Silbey, Annu. Rev. Phys. Chem. 55, 457 (2004).
  3. M. Orrit, Single Mol. 3, 255 (2002).
  4. W. P. Ambrose and W. E. Moerner, Nature (London) 349, 225 (1991).
  5. K. D. Weston, P. J. Carson, H. Metiu, and S. K. Buratto, J. Chem. Phys. 109, 7474 (1998).
  6. J. K. Trautman and J. J. Macklin, Chem. Phys. 205, 221 (1996).
  7. R. M. Dickson, A. B. Cubitt, R. Y. Tsien, and W. E. Moerner, Nature (London) 388, 355 (1997).
  8. D. A. Vanden Bout, W. -T. Yip, D. Hu, D. -K. Fu, T. M. Swager, and P. F. Barbara, Science 277, 1074 (1997).
  9. L. Fleury, J. -M. Segura, G. Zumofen, B. Hecht, and U. P. Wild, Phys. Rev. Lett. 84, 1148 (2000).
  10. M. Haase, C. G. Hubner, E. Reuther, A. Herrmann, K. Mullen, and T. Basche, J. Phys. Chem. B 108, 10445 (2004).
  11. M. W. Holman, R. C. Liu, and D. M. Adams, J. Am. Chem. Soc. 125, 12649 (2003).
  12. X. S. Xie, P. J. Choi, G. W. Li, N. K. Lee, and G. Lia, Annu. Rev. Biophys. Biomol. Struct. 37, 417 (2008).
  13. F. Cichos, C. von Borczyskowski, and M. Orrit, Curr. Opin. Colloid Interface Sci. 12, 272 (2007).
  14. M. Heilemann, P. Dedecker, J. Hofkens, and M. Sauer, Laser Photonics Rev. 3, 180 (2009).
  15. W. E. Moerner and D. P. Fromm, Rev. Sci. Instrum. 74, 3597 (2003).
  16. L. Zhang, S. Aite, and Z. Yu, Rev. Sci. Instrum. 78, 083701 (2007).
  17. J. -M. Segura, A. Renn, and B. Hecht, Rev. Sci. Instrum. 71, 1706 (2000).
  18. M. Born and E. Wolf, Principles of Optics: Electromagnetic Theory of Propagation, Interference, and Diffraction of Light (Pergamon, New York, 1980).
  19. O. L. A. Monti, T. A. Baker, and D. J. Nesbitt, J. Chem. Phys. 125, 154709 (2006).
  20. K. Zhang, H. Chang, A. Fu, A. P. Alivisatos, and H. Yang, Nano Lett. 6, 843 (2006).
  21. L. P. Watkins and H. Yang, J. Phys. Chem. B 109, 617 (2005).
  22. A. Diaspro, Confocal and Two-Photon Microscopy: Foundations, Applications, and Advances, 1st ed. (Wiley, New York, 2001).
  23. A. M. Herrero, B. P. Gila, C. R. Abernathy, S. J. Pearton, V. Craciun, K. Siebein, and F. Ren, Appl. Phys. Lett. 89, 092117 (2006).
  24. M. Kuno, D. P. Fromm, H. F. Hamann, A. Gallagher, and D. J. Nesbitt, J. Chem. Phys. 115, 1028 (2001).

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