Apparatus for single ice crystal growth from the melt
Rev. Sci. Instrum. 80, 115102 (2009); doi:10.1063/1.3222739
Published 3 November 2009
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A crystal growth apparatus was designed and built to study the effect of growth modifiers, antifreeze proteins and antifreeze glycoproteins (AFGPs), on ice crystal growth kinetics and morphology. We used a capillary growth technique to obtain a single ice crystal with well-defined crystallographic orientation grown in AFGP solution. The basal plane was readily observed by rotation of the capillary. The main growth chamber is approximately a 0.8 ml cylindrical volume. A triple window arrangement was used to minimize temperature gradients and allow for up to 10 mm working distance objective lens. Temperature could be established to within ±10 mK in as little as 3.5 min and controlled to within ±2 mK after 15 min for at least 10 h. The small volume growth chamber and fast equilibration times were necessary for parabolic flight microgravity experiments. The apparatus was designed for use with inverted and side mount configurations.
©2009 American Institute of Physics
| History: | Received 16 April 2009; accepted 16 August 2009; published 3 November 2009 |
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http://link.aip.org/link/?RSINAK/80/115102/1 |
KEYWORDS and PACS
capillarity,
crystal growth from solution,
crystal morphology,
crystal orientation,
freezing,
ice,
lenses,
molecular biophysics,
nucleation,
proteins,
temperature control,
zero gravity experiments
- 81.10.Dn
Crystal growth from solutions - 82.37.Rs
Single molecule manipulation of proteins and other biological molecules (chemical kinetics) - 87.14.E-
Proteins - 64.60.Q-
Nucleation in phase transitions - 82.39.-k
Chemical kinetics in biological systems - 81.10.Mx
Crystal growth in microgravity environments - YEAR: 2009
PUBLICATION DATA
0034-6748 (print)
1089-7623 (online)
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