Multicomponent protein patterning of material surfaces

Micro- and nano-scale protein patterns have gained significant technological interest. While certain techniques for single-component protein patterning are well-established, multicomponent protein patterning approaches are a current topic of intensive research, which might enable complex biosensor systems and expand the knowledge in protein-protein and protein-cell or cell-cell interactions. Only a few patterning methods are suitable for the realization of three dimensional patterns, which are essential for many applications e.g. in the design of scaffolds for regenerative therapies. In this feature article representative approaches for creating multicomponent protein patterning are presented and their potential for tailoring microenvironments for cells on biomaterials surfaces is discussed. ©2010