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Numerical investigation of flow-through immunoassay in a microchannel

Source: J. Appl. Phys. 107, 034907 (2010); doi:10.1063/1.3284077

Published 9 February 2010

KEYWORDS and PACS
Keywords
PACS
  • 87.80.Ek
    Mechanical and micromechanical techniques (biophysical research methods)
  • 87.85.Rs
    Nanotechnologies - applications in biomedical engineering
  • 85.85.+j
    Micro- and nano-electromechanical systems (MEMS/NEMS) and devices
  • 75.50.Tt
    Fine-particle magnetic systems; nanocrystalline materials
  • YEAR: 2010
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PUBLICATION DATA
ISSN:
1553-9628 (online)
Publisher:
AIP is a member of CrossRef AIP
A Sinha,1 R Ganguly,2 and I K. Puri1
1Department of Engineering Science and Mechanics, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061, USA
2Department of Power Engineering, Jadavpur University, Kolkata 700098, India
Immunomagnetic separation (IMS) is a method to isolate biomaterials from a host fluid in which specifically selected antibodies attached to magnetic particles bind with their corresponding antigens on the surface of the target biological entities. A magnet separates these entities from the fluid through magnetophoresis. The method has promising applications in microscale biosensors. We develop a comprehensive model to characterize the interaction between target species and magnetic particles in microfluidic channels. The mechanics of the separation of target nonmagnetic N particles by magnetic M particles are investigated using a particle dynamics simulation. We consider both interparticle magnetic interactions and the binding of the functionalizing strands of complementary particles. The temporal growth of a particle aggregate and the relative concentrations of M and N particles are investigated under different operating conditions. A particle aggregate first grows and then exhibits periodic washaway about a quasisteady mean size. The washaway frequency and amplitude depend on the initial fractional concentration of N particles while the aggregate size scales linearly with the dipole strength and inversely with the fluid flow rate. ©2010 American Institute of Physics
History: Received 20 June 2009; accepted 7 December 2009; published 9 February 2010
Permalink: http://link.aip.org/link/?JAPIAU/107/034907/1

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