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Application of intravital microscopy in studies of tumor microcirculation

Source: J. Biomed. Opt. 15, 011113 (2010); doi:10.1117/1.3281674

Published 9 February 2010

KEYWORDS and PACS
Keywords
PACS
  • 87.64.M-
    Optical microscopy in biophysics and medical physics
  • 87.19.U-
    Haemodynamics
  • 87.19.rh
    Fluid transport and rheology in tissues and organs (higher organisms)
  • YEAR: 2010
PUBLICATION DATA
ISSN:
1553-9628 (online)
Publisher:
AIP is a member of CrossRef SPIE
Sarah Jane Lunt
University of Sheffield, School of Medicine, Tumour Microcirculation Group and Department of Oncology, Beech Hill Road, Sheffield, S10 2RX, United Kingdom

Colin Gray
University of Sheffield, School of Medicine, Department of Cardiovascular Science, Beech Hill Road, Sheffield, S10 2RX, United Kingdom

Constantino Carlos Reyes-Aldasoro
University of Sheffield, School of Medicine, Tumour Microcirculation Group and Department of Oncology, Beech Hill Road, Sheffield, S10 2RX, United Kingdom

Stephen J. Matcher
University of Sheffield, The Kroto Institute, Broad Lane, Sheffield, S3 7HQ, United Kingdom

Gillian M. Tozer
University of Sheffield, School of Medicine, Tumour Microcirculation Group and Department of Oncology, Beech Hill Road, Sheffield, S10 2RX, United Kingdom
To grow and progress, solid tumors develop a vascular network through co-option and angiogenesis that is characterized by multiple structural and functional abnormalities, which negatively influence therapeutic outcome through direct and indirect mechanisms. As such, the morphology and function of tumor blood vessels, plus their response to different treatments, are a vital and active area of biological research. Intravital microscopy (IVM) has played a key role in studies of tumor angiogenesis, and ongoing developments in molecular probes, imaging techniques, and postimage analysis methods have ensured its continued and widespread use. In this review we discuss some of the primary advantages and disadvantages of IVM approaches and describe recent technological advances in optical microscopy (e.g., confocal microscopy, multiphoton microscopy, hyperspectral imaging, and optical coherence tomography) with examples of their application to studies of tumor angiogenesis. ©2010 Society of Photo-Optical Instrumentation Engineers
History: Received 11 September 2009; revised 20 October 2009; accepted 22 October 2009; published 9 February 2010
Permalink: http://dx.doi.org/10.1117/1.3281674

REFERENCES (141)

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  1. B. S. Sorg, M. E. Hardee, N. Agarwal, B. J. Moeller, and M. W. Dewhirst, “Spectral imaging facilitates visualization and measurements of unstable and abnormal microvascular oxygen transport in tumors,” J. Biomed. Opt. 13(1), 014026 (2008).
  2. T. P. Thomas, J. Y. Ye, Y. C. Chang, A. Kotlyar, Z. Cao, I. J. Majoros, T. B. Norris, and J. R. Baker, “Investigation of tumor cell targeting of a dendrimer nanoparticle using a double-clad optical fiber probe,” J. Biomed. Opt. 13(1), 014024 (2008).
  3. P. Kim, M. Puoris"haag, D. Cote, C. P. Lin, and S. H. Yun, “In vivo confocal and multiphoton microendoscopy,” J. Biomed. Opt. 13(1), 010501 (2008).
  4. B. S. Sorg, B. J. Moeller, O. Donovan, Y. Cao, and M. W. Dewhirst, “Hyperspectral imaging of hemoglobin saturation in tumor microvasculature and tumor hypoxia development,” J. Biomed. Opt. 10(4), 44004 (2005).

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