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Label-free discrimination of normal and pulmonary cancer tissues using multiphoton fluorescence ratiometric microscopy

Source: Appl. Phys. Lett. 97, 043706 (2010); doi:10.1063/1.3460913

Published 29 July 2010 | See: Erratum

ERRATUM
  1. Erratum: “Label-free discrimination of normal and pulmonary cancer tissues using multiphoton fluorescence ratiometric microscopy” [Appl. Phys. Lett. 97, 043706 (2010)]
    Chun-Chin Wang (王俊欽) et al.
    Appl. Phys. Lett. 97, 239904 (2010)
KEYWORDS and PACS
Keywords
PACS
  • 87.64.mn
    Multiphoton microscopy in biophysics and medical physics
  • 87.64.kv
    Fluorescence spectroscopy in biophysics and medical physics
  • 87.50.wf
    Biophysical mechanisms of interaction (optical/infrared radiation)
  • 87.19.xj
    Cancer
  • YEAR: 2010
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PUBLICATION DATA
ISSN:
1553-9628 (online)
Publisher:
AIP is a member of CrossRef AIP
Chun-Chin Wang (王俊欽),1 Ruei-Jr Wu (吳瑞智),2 Sung-Jan Lin (林頌然),1,3 Yang-Fang Chen (陳永芳),2 and Chen-Yuan Dong (董成淵)2
1Institute of Biomedical Engineering, National Taiwan University, Taipei 106, Taiwan
2Department of Physics, National Taiwan University, Taipei 106, Taiwan
3Department of Dermatology, National Taiwan University Hospital, Taipei 100, Taiwan
We performed multiphoton excited autofluorescence and second harmonic generation microscopy for the distinction of normal, lung adenocarcinoma (LAC), and squamous cell carcinoma (SCC) specimens. In addition to morphological distinction, we derived quantitative metrics of cellular redox ratios for cancer discrimination. Specifically, the redox ratios of paired normal/SCC and normal/LAC specimens were found to be 0.53±0.05/0.41±0.06 and 0.56±0.02/0.35±0.06, respectively. The lower redox ratios in cancer specimens, indicating an increase in metabolic activity. These results show that the combination of morphological multiphoton imaging along with redox ratio indices can be used for the discrimination of normal and pulmonary cancer tissues. ©2010 American Institute of Physics
History: Received 14 April 2010; accepted 14 June 2010; published 29 July 2010
Permalink: http://link.aip.org/link/?APPLAB/97/043706/1

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