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Changes of collagen and nicotinamide adenine dinucleotide in human cancerous and normal prostate tissues studied using native fluorescence spectroscopy with selective excitation wavelength

Source: J. Biomed. Opt. 15, 047008 (2010); doi:10.1117/1.3463479

Published 23 July 2010

EDITORIALLY RELATED
  1. Errata: Changes of collagen and nicotinamide adenine dinucleotide in human cancerous and normal prostate tissues studied using native fluorescence spectroscopy with selective excitation wavelength
    Yang Pu et al.
    J. Biomed. Opt. 15, 049801 (2010)
KEYWORDS and PACS
Keywords
PACS
  • 87.64.kv
    Fluorescence spectroscopy in biophysics and medical physics
  • 87.15.M-
    Spectra of biomolecules
  • 87.85.-d
    Biomedical engineering
  • 87.14.E-
    Proteins
  • YEAR: 2010
PUBLICATION DATA
ISSN:
1553-9628 (online)
Publisher:
AIP is a member of CrossRef SPIE
Yang Pu
City College of the City University of New York, Institute for Ultrafast Spectroscopy and Lasers, Departments of Electrical Engineering, 160 Convent Avenue, New York, New York 10031

Wubao Wang, Guichen Tang, and Robert R. Alfano
City College of the City University of New York, Institute for Ultrafast Spectroscopy and Lasers, Department of Physics, 160 Convent Avenue, New York, New York 10031
The fluorescence spectra of human cancerous and normal prostate tissues obtained by the selective excitation wavelength of 340  nm were measured. The contributions of principle biochemical components to tissue fluorescence spectra were investigated using the method of multivariate curve resolution with alternating least squares. The results show that there is a reduced contribution from the emission of collagen and increased contribution from nicotinamide adenine dinucleotide (NADH) in cancerous tissues as compared with normal tissue. This difference is attributed to the changes of relative contents of NADH and collagen during cancer development. This research may present a potential native biomarker for prostate cancer detection. ©2010 Society of Photo-Optical Instrumentation Engineers
History: Received 5 March 2010; revised 19 May 2010; accepted 21 May 2010; published 23 July 2010; corrected 30 July 2010
Permalink: http://dx.doi.org/10.1117/1.3463479

REFERENCES (12)

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  1. R. Drezek, K. Sokolov, U. Utzinger, I. Boiko, A. Malpica, M. Follen, and R. Richards-Kortum, “Understanding the contributions of NADH and collagen to cervical tissue fluorescence spectra: modeling, measurements, and implications,” J. Biomed. Opt. 6(4), 385–396 (2001).
  2. I. Zeylikovich, G. C. Tang, A. Katz, Y. Budansky, and R. R. Alfano, “Fluorescence-based micro-endoscopes for breast cancer ductoscopy,” Proc. SPIE 6091, 609102 (2006).

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