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Gigaohm resistance membrane seals with stealth probe electrodes

Source: Appl. Phys. Lett. 97, 033704 (2010); doi:10.1063/1.3464954

Published 21 July 2010

KEYWORDS and PACS
Keywords
PACS
  • 87.85.Rs
    Nanotechnologies - applications in biomedical engineering
  • 87.85.-d
    Biomedical engineering
  • 87.19.R-
    Mechanical and electrical properties of tissues and organs (higher organisms)
  • YEAR: 2010
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PUBLICATION DATA
ISSN:
1553-9628 (online)
Publisher:
AIP is a member of CrossRef AIP
Piyush Verma and Nicholas A. Melosh
Department of Materials Science and Engineering, Stanford University, Stanford, California 94305, USA
Direct electrical access into the cell interior is required for low-noise recording of ion channel activity, yet conventional patch clamp techniques are destructive, leading to rapid cell death, while on-chip devices have poor seal resistances. Here we report chip-based nanoscale electrodes that nondestructively incorporate into biological membranes. These consist of a metallic post with a hydrophobic band that mimics transmembrane proteins, driving insertion into the lipid membrane and forming a tight seal at the electrode-membrane interface. We demonstrate spontaneous gigaohm seals with an average seal resistance of 3.8±1.9  GOmega using red blood cells, and show the nanoband is the key attribute for high resistances. ©2010 American Institute of Physics
History: Received 2 June 2010; accepted 22 June 2010; published 21 July 2010
Permalink: http://link.aip.org/link/?APPLAB/97/033704/1

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