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Photoacoustic lifetime imaging of dissolved oxygen using methylene blue

Source: J. Biomed. Opt. 15, 040501 (2010); doi:10.1117/1.3465548

Published 21 July 2010

KEYWORDS and PACS
Keywords
PACS
  • 87.63.L-
    Visual medical imaging
  • 87.50.wf
    Biophysical mechanisms of interaction (optical/infrared radiation)
  • 87.19.xj
    Cancer
  • 43.35.Ud
    Thermoacoustics, high temperature acoustics, photoacoustic effect
  • 42.62.Be
    Biological and medical applications of lasers
  • YEAR: 2010
PUBLICATION DATA
ISSN:
1553-9628 (online)
Publisher:
AIP is a member of CrossRef SPIE
Shai Ashkenazi
University of Minnesota, Department of Biomedical Engineering, 312 Church Street Southeast, Minneapolis, Minnesota 55455
Measuring distribution of dissolved oxygen in biological tissue is of prime interest for cancer diagnosis, prognosis, and therapy optimization. Tumor hypoxia indicates poor prognosis and resistance to radiotherapy. Despite its major clinical significance, no current imaging modality provides direct imaging of tissue oxygen. We present preliminary results demonstrating the potential of photoacoustic lifetime imaging (PALI) for noninvasive, 3-D imaging of tissue oxygen. The technique is based on photoacoustic probing of the excited state lifetime of methylene blue (MB) dye. MB is an FDA-approved water soluble dye with a peak absorption at 660  nm. A double pulse laser system (pump probe) is used to excite the dye and probe its transient absorption by detecting photoacoustic emission. The relaxation rate of MB depends linearly on oxygen concentration. Our measurements show high photoacoustic signal contrast at a probe wavelength of 810  nm, where the excited state absorption is more than four times higher than the ground state absorption. Imaging of a simple phantom is demonstrated. We conclude by discussing possible implementations of the technique in clinical settings and combining it with photodynamic therapy (PDT) for real-time therapy monitoring. ©2010 Society of Photo-Optical Instrumentation Engineers
History: Received 8 April 2010; revised 23 May 2010; accepted 23 June 2010; published 21 July 2010
Permalink: http://dx.doi.org/10.1117/1.3465548

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