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Integration of plasma-assisted surface chemical modification, soft lithography, and protein surface activation for single-cell patterning

Source: Appl. Phys. Lett. 97, 043705 (2010); doi:10.1063/1.3462326

Published 28 July 2010

KEYWORDS and PACS
Keywords
PACS
  • 87.17.-d
    Cell processes
  • 87.85.J-
    Biomaterials (biomedical engineering)
  • 87.15.R-
    Biochemical reactions and kinetics
  • YEAR: 2010
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PUBLICATION DATA
ISSN:
1553-9628 (online)
Publisher:
AIP is a member of CrossRef AIP
Q. Cheng and K. Komvopoulos
Department of Mechanical Engineering, University of California, Berkeley, California 94720, USA
Surface patterning for single-cell culture was accomplished by combining plasma-assisted surface chemical modification, soft lithography, and protein-induced surface activation. Hydrophilic patterns were produced on Parylene C films deposited on glass substrates by oxygen plasma treatment through the windows of polydimethylsiloxane shadow masks. After incubation first with Pluronic F108 solution and then serum medium overnight, surface seeding with mesenchymal stem cells in serum medium resulted in single-cell patterning. The present method provides a means of surface patterning with direct implications in single-cell culture. ©2010 American Institute of Physics
History: Received 1 May 2010; accepted 17 June 2010; published 28 July 2010
Permalink: http://link.aip.org/link/?APPLAB/97/043705/1

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