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New methodology to assess activity status of occlusal caries in primary teeth using laser fluorescence device

Source: J. Biomed. Opt. 15, 047005 (2010); doi:10.1117/1.3463007

Published 29 July 2010

KEYWORDS and PACS
Keywords
PACS
  • 87.63.lt
    Laser imaging in medicine
  • 42.62.Be
    Biological and medical applications of lasers
  • YEAR: 2010
PUBLICATION DATA
ISSN:
1553-9628 (online)
Publisher:
AIP is a member of CrossRef SPIE
Mariana Minatel Braga
Departmento de Ortodontia e Odontopediatria, Faculdade de Odontologia da São Paulo, São Paulo, Brazil 05508-000

Monique Saveriano de Benedetto
Universidade de São Paulo, Faculdade de Odontologia, São Leopoldo Mandic, Brazil 13100-000

Jose Carlos Pettorossi Imparato and Fausto Medeiros Mendes
Universidade de São Paulo, Departmento de Ortodontia e Odontopediatria, Faculdade de Odontologia da São Paulo, São Paulo, Brazil 05508-000
An in vivo study was conducted to verify the ability of laser fluorescence (LF) to assess the activity status of occlusal caries in primary teeth, using different air-drying times. Occlusal sites (707) were examined using LF (DIAGNOdent) after air-drying for 3  s and 15  s, and the difference between readings (DIF15  s–3  s) was calculated. For concurrent validation of LF, visual criteria–Nyvad (NY) and Lesion Activity Assessment associated with the International Caries Detection and Assessment System (LAA-ICDAS)–were the reference standards for lesion activity. Histological exam using a pH-indicator dye (0.1% methyl red) was performed in 46 exfoliated/extracted teeth for criterion validation. LF readings and DIF15  s–3  s were compared using Kruskall-Wallis and Mann-Whitney tests. Receiver operating characteristic analyses were performed and validity parameters calculated, considering the caries activity assessment. Using NY, active lesions (3  s: 30.0±29.3; 15  s: 34.2±30.6) presented higher LF readings than inactive lesions (3  s: 17.0±16.3; 15  s: 19.2±17.3; p<0.05), different from LAA-ICDAS. Active cavitated caries resulted in higher LF readings (3  s: 50.3±3.5; 15  s: 54.7±30.2) than inactive cavitated caries (3  s: 19.9±16.3; 15  s: 22.8±16.8). Therefore, LF can distinguish cavitated active and inactive lesions classified by NY, but not by LAA-ICDAS; however, this difference might be related to the visual system rather than to LF. The air-drying time could be an alternative to improve the caries activity assessment; however, longer air-drying time is suggested to be tested subsequently. ©2010 Society of Photo-Optical Instrumentation Engineers
History: Received 12 February 2010; revised 7 May 2010; accepted 17 May 2010; published 29 July 2010
Permalink: http://dx.doi.org/10.1117/1.3463007

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