Home | About Journal | Web Links | E-mail Alerts | RSS RSS Icon | Browse
Previous Article Next Article

Needle-compatible single fiber bundle image guide reflectance endoscope

Source: J. Biomed. Opt. 15, 040502 (2010); doi:10.1117/1.3465558

Published 29 July 2010

KEYWORDS and PACS
Keywords
PACS
  • 87.64.M-
    Optical microscopy in biophysics and medical physics
  • 87.63.L-
    Visual medical imaging
  • 87.85.Pq
    Biomedical imaging
  • 87.17.-d
    Cell processes
  • 87.50.wf
    Biophysical mechanisms of interaction (optical/infrared radiation)
  • YEAR: 2010
PUBLICATION DATA
ISSN:
1553-9628 (online)
Publisher:
AIP is a member of CrossRef SPIE
Jiantang Sun,1 Chenghao Shu,1 Benjamin Appiah,2 and Rebekah Drezek1,2
1Rice University, Department of Bioengineering, 6100 Main Street, Houston, Texas 77005
2Rice University, Department of Electrical and Computer Engineering, 6100 Main Street, Houston, Texas 77005
We developed a miniaturized high-resolution low-cost reflectance-mode fiber microscope (RFM) aimed at optical tissue biopsy applications using a polarized imaging configuration to suppress background noise from specular reflectance. The RFM is equipped with an air-cooled light-emitting-diode illumination module and a single 450-µm outer-diameter fiber bundle image guide compatible with a 20-gauge needle. The dual illumination and image acquisition nature of the fiber bundle simplifies the system and reduces the total cost. Imaging tests with a United States Air Force resolution target demonstrate a lateral resolution of ~3.5  µm. The performance of the imaging system is evaluated by examining samples of cells and excised human tissue. Still and video rate images are obtainable in reflectance mode using intrinsic contrast. ©2010 Society of Photo-Optical Instrumentation Engineers
History: Received 26 April 2010; revised 11 June 2010; accepted 23 June 2010; published 29 July 2010
Permalink: http://dx.doi.org/10.1117/1.3465558

REFERENCES (10)

  1. C. Liang, M. Descour, K. B. Sun, and R. Richards-Kortum, “Fiber confocal reflectance microscope (FCRM) for in-vivo imaging,” Opt. Express 9, 821–830 (2001). [MEDLINE]
  2. K. C. Maitland, A. M. Gillenwater, M. D. Williams, A. K. El-Naggar, M. R. Descour, and R. R. Richards-Kortum, “In vivo imaging of oral neoplasia using a miniaturized fiber optic confocal reflectance microscope,” Oral Oncol. 44, 1059–1066 (2008).
  3. F. Jean, G. Bourg-Heckly, and B. Viellerobe, “Fibered confocal spectroscopy and multicolor imaging system for in vivo fluorescence analysis,” Opt. Express 15, 4008–4017 (2007). [MEDLINE]
  4. V. Dubaj, A. Mazzolini, A. Wood, and M. Harris, “Optic fiber bundle contact imaging probe employing a laser scanning confocal microscope,” J. Microsc. 207, 108–117 (2002). [MEDLINE]
  5. R. Kiesslich, M. Goetz, M. Vieth, P. R. Galle, and M. F. Neurath, “Technology Insight: confocal laser endoscopy for in vivo diagnosis of colorectal cancer,” Nat. Clin. Pract. Oncol. 4, 480–490 (2007).
  6. T. J. Muldoon, M. C. Pierce, D. L. Nida, M. D. Williams, A. Gillenwater, and R. Richards-Kortum, “Subcellular-resolution molecular imaging within living tissue by fiber microendoscopy,” Opt. Express 15, 16413–16423 (2007). [MEDLINE]
  7. T. J. Muldoon, S. Anandasabapathy, D. Maru, and R. Richards-Kortum, “High-resolution imaging in Barrett's esophagus: a novel, low-cost endoscopic microscope,” Gastrointest. Endosc. 68, 737–744 (2008). [MEDLINE]
  8. T. J. Muldoon, N. Thekkek, D. Roblyer, D. Maru, N. Harpaz, J. Potack, S. Anandasabapathy, and R. Richards-Kortum, “Evaluation of quantitative image analysis criteria for the high-resolution microendoscopic detection of neoplasia in Barrett's esophagus,” J. Biomed. Opt. 15, 026027 (2010). [MEDLINE]
  9. Sumitomo Electric U.S.A., Image Guide Technical Document <http://www.sumitomoelectricusa.com/scripts/products/ofig/image.cfm> (2010).
  10. A. G. von Arnim, X. W. Deng, and M. G. Stacey, “Cloning vectors for the expression of green fluorescent protein fusion proteins in transgenic plants,” Gene 221, 35–43 (1998). [MEDLINE]




ADVERTISEMENT