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Note: Microelectromechanical systems Coulter counter for cell monitoring and counting

Source: Rev. Sci. Instrum. 81, 076103 (2010); doi:10.1063/1.3462327

Published 19 July 2010

KEYWORDS and PACS
Keywords
PACS
  • 87.16.-b
    Subcellular structure and processes
  • 87.85.-d
    Biomedical engineering
  • 07.10.Cm
    Micromechanical devices and systems
  • YEAR: 2010
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PUBLICATION DATA
ISSN:
1553-9628 (online)
Publisher:
AIP is a member of CrossRef AIP
Yifan Wu,1 James D. Benson,2 John K. Critser,3 and Mahmoud Almasri1
1Department of Electrical and Computer Engineering, University of Missouri, Columbia, Missouri 65211, USA
2Department of Mathematics, University of Missouri, Columbia, Missouri 65211, USA
3Comparative Medicine Center, College of Veterinary Medicine, University of Missouri, Columbia, Missouri 65211, USA
This note describes the design, fabrication, and testing of a novel microelectromechanical systems Coulter counter. The Coulter counter will be used to detect and monitor impedance changes of cells as a function of time in response to different experimental extracellular environments. The device consists of SU-8 (negative photoresist) microchannels, vertical electroplated electrodes, polydimethylsiloxane cover, and is divided into a passive mixing region, a focusing region using negative dielectrophoretic forces, and a measuring region defined by multiple electroplated electrode pairs. The devices were tested using both microbeads in saline water and fibroblast cells in phosphate buffered saline solution. The results show that the proposed microsystem is capable of monitoring impedance of cells at different positions along the Coulter microchannel. ©2010 American Institute of Physics
History: Received 19 February 2010; accepted 17 June 2010; published 19 July 2010
Permalink: http://link.aip.org/link/?RSINAK/81/076103/1

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