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Development of a multitarget tracking system for paramecia

Source: Rev. Sci. Instrum. 81, 074302 (2010); doi:10.1063/1.3460266

Published 21 July 2010

KEYWORDS and PACS
Keywords
PACS
  • 87.17.Jj
    Cell locomotion; chemotaxis
  • 89.20.Ff
    Computer science and technology
  • YEAR: 2010
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PUBLICATION DATA
ISSN:
1553-9628 (online)
Publisher:
AIP is a member of CrossRef AIP
Yu-Sing Yeh,1 Ke-Nung Huang,2 Sun-Lon Jen,2 Yan-Chay Li,3 and Ming-Shing Young1
1Department of Electrical Engineering, National Cheng Kung University, Tainan, 701 Taiwan
2Department of Electronic Engineering, I-Shou University, Kaohsiung, 840 Taiwan
3Department of Biomedical Engineering, I-Shou University, Kaohsiung, 840 Taiwan
This investigation develops a multitarget tracking system for the motile protozoa, paramecium. The system can recognize, track, and record the orbit of swimming paramecia within a 4 mm diameter of a circular experimental pool. The proposed system is implemented using an optical microscope, a charge-coupled device camera, and a software tool, Laboratory Virtual Instrumentation Engineering Workbench (LABVIEW). An algorithm for processing the images and analyzing the traces of the paramecia is developed in LABVIEW. It focuses on extracting meaningful data in an experiment and recording them to elucidate the behavior of paramecia. The algorithm can also continue to track paramecia even if they are transposed or collide with each other. The experiment demonstrates that this multitarget tracking design can really track more than five paramecia and simultaneously yield meaningful data from the moving paramecia at a maximum speed of 1.7 mm/s. ©2010 American Institute of Physics
History: Received 4 January 2010; accepted 14 June 2010; published 21 July 2010
Permalink: http://link.aip.org/link/?RSINAK/81/074302/1

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