Vir. J. Bio. Phys. Res. / Volume 20 / Issue 3 / INSTRUMENTATION DEVELOPMENT

Vortex-assisted DNA delivery

Source: Lab Chip 10, 2057 (2010); doi:10.1039/c004472e

Issue Date: 1 August 2010

ABSTRACT

^* Full Text: Access via Source Journal Abstract

PUBLICATION DATA

ISSN:

1553-9628 (online)

Publisher:

RSC

Jun Wang
Birck Nanotechnology Center, Purdue University, West Lafayette, Indiana 47907, USA

Yihong Zhan

Victor M. Ugaz

Chang Lu

Electroporationis one of the most widely used methods to deliverexogenous DNA payloads into cells, but a major limitation isthat only a small fraction of the total membrane surfaceis permeabilized. Here we show how this barrier can beeasily overcome by harnessing hydrodynamic effects associated with Dean flowsthat occur along curved paths. Under these conditions, cells aresubjected to a combination of transverse vortex motion and rotationthat enables the entire membrane surface to become uniformly permeabilized.Greatly improved transfection efficiencies are achievable with only a simplemodification to the design of existing continuous flow electroporation systems. ©2010

Permalink:

http://dx.doi.org/10.1039/c004472e

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Vortex-assisted DNA delivery

Source: Lab Chip 10, 2057 (2010); doi:10.1039/c004472e