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Study of back-scattering microspectrum for stomach cells at single-cell scale

Source: J. Biomed. Opt. 15, 040505 (2010); doi:10.1117/1.3469782

Published 11 August 2010

KEYWORDS and PACS
Keywords
PACS
  • 87.64.Cc
    Scattering of visible, uv, and infrared radiation (spectroscopic/microscopic techniques in biophysics/medical physics)
  • 87.19.xj
    Cancer
  • 87.64.M-
    Optical microscopy in biophysics and medical physics
  • YEAR: 2010
PUBLICATION DATA
ISSN:
1553-9628 (online)
Publisher:
AIP is a member of CrossRef SPIE
Cheng Wang,1 Xudong Guo,1 Baoying Fang,2 and Chengli Song1
1University of Shanghai for Science and Technology, School of Medical Instrument and Food Engineering, Shanghai, China
2University of Shanghai for Science and Technology, School of Optical-Electrical and Computer Engineering, Shanghai, China
A fiber confocal backscattering (FCBS) spectrometer is developed based on fiber confocal microscopy and light scattering theory. The FCBS spectrometer can provide imaging and spectral information simultaneously at the cellular scale. Normal stomach epithelial cell line GES-1 and cancerous cell line NCI-N87 are measured and their spectral results show that backscattering intensity from NCI-N87 cells is stronger than that from GES-1 cells in 500  to  800  nm, and the GES-1 cells scattering spectra show regular intensity changes, while the NCI-N87 cells do not. The experiments prove that the FCBS spectrometer is able to distinguish cancerous cells from normal stomach cells at the cellular level. The spectrometer could be further developed into a noninvasive optical technology for early cancer detection. ©2010 Society of Photo-Optical Instrumentation Engineers
History: Received 24 December 2009; revised 21 June 2010; accepted 1 July 2010; published 11 August 2010
Permalink: http://dx.doi.org/10.1117/1.3469782

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