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Three-dimensional optical method for integrated visualization of mouse islet microstructure and vascular network with subcellular-level resolution

Source: J. Biomed. Opt. 15, 046018 (2010); doi:10.1117/1.3470241

Published 4 August 2010

KEYWORDS and PACS
Keywords
PACS
  • 87.64.M-
    Optical microscopy in biophysics and medical physics
  • 87.19.Hh
    Cardiac dynamics
  • 87.17.-d
    Cell processes
  • 87.64.Cc
    Scattering of visible, uv, and infrared radiation (spectroscopic/microscopic techniques in biophysics/medical physics)
  • 87.57.cf
    Spatial resolution (medical image quality)
  • YEAR: 2010
PUBLICATION DATA
ISSN:
1553-9628 (online)
Publisher:
AIP is a member of CrossRef SPIE
Ya-Yuan Fu and Chih-Hsuan Lu
National Tsing Hua University, Department of Chemical Engineering, Hsinchu, Taiwan 30013

Chi-Wen Lin
National Tsing Hua University, Institute of Biotechnology, Hsinchu, Taiwan 30013

Jyuhn-Huarng Juang
Chang Gung University and Memorial Hospital, Department of Internal Medicine, Division of Endocrinology and Metabolism, Taoyuan, Taiwan 33302

Grigori Enikolopov
Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724

Eric Sibley
Stanford University, School of Medicine, Division of Pediatric Gastroenterology, Stanford, California 94305

Ann-Shyn Chiang
National Tsing Hua University, Institute of Biotechnology, Hsinchu, Taiwan 30013

Shiue-Cheng Tang
National Tsing Hua University, Department of Chemical Engineering, Hsinchu, Taiwan 30013
Microscopic visualization of islets of Langerhans under normal and diabetic conditions is essential for understanding the pathophysiology of the disease. The intrinsic opacity of pancreata, however, limits optical accessibility for high-resolution light microscopy of islets in situ. Because the standard microtome-based, 2-D tissue analysis confines visualization of the islet architecture at a specific cut plane, 3-D representation of image data is preferable for islet assessment. We applied optical clearing to minimize the random light scattering in the mouse pancreatic tissue. The optical-cleared pancreas allowed penetrative, 3-D microscopic imaging of the islet microstructure and vasculature. Specifically, the islet vasculature was revealed by vessel painting—lipophilic dye labeling of blood vessels—for confocal microscopy. The voxel-based confocal micrographs were digitally processed with projection algorithms for 3-D visualization. Unlike the microtome-based tissue imaging, this optical method for penetrative imaging of mouse islets yielded clear, continuous optical sections for an integrated visualization of the islet microstructure and vasculature with subcellular-level resolution. We thus provide a useful imaging approach to change our conventional planar view of the islet structure into a 3-D panorama for better understanding of the islet physiology. ©2010 Society of Photo-Optical Instrumentation Engineers
History: Received 6 March 2010; revised 2 June 2010; accepted 3 June 2010; published 4 August 2010
Permalink: http://dx.doi.org/10.1117/1.3470241

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