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Measurement of the ratio of forward-propagating to back-propagating second harmonic signal using a single objective

Source: Opt. Express 18, 10538 (2010); doi:10.1364/OE.18.010538

Issue Date: 15 August 2010

PACS
  • 87.57.-s
    Medical imaging
  • 87.85.M-
    Biotechnology
  • 87.64.-t
    Spectroscopic and microscopic techniques in biophysics and medical physics
  • 42.66.-p
    Physiological optics
  • 87.63.-d
    Medical non-ionizing radiation equipment and techniques
  • 07.60.Pb
    Conventional optical microscopes
  • 07.70.-v
  • YEAR: 2010
PUBLICATION DATA
ISSN:
1553-9628 (online)
Publisher:
AIP is a member of CrossRef OSA
In this paper, we present a method to determine, for the first time, the ratio of forward-propagating second harmonic (SHG) signal to back-propagating SHG signal (F/B) in vivo on the surface of intact tissue samples without any biopsy or tissue sectioning, using only epidetection (i.e., via a single objective lens). This method has the additional benefit of using the confocal detection apparatus already contained within common commercially available two-photon laser-scanning microscopes, and hence can allow the measurement of the SHG F/B ratio in vivo with minimal purchase of new equipment. ©2010 Optical Society of America

(As supplied by publisher.)

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